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Smart IP

Smart IP:  Fast and Effective IP

Smart-IP is a line of highly specific tag antibodies conjugated to magnetic beads or magnetic agarose beads designed for faster immunoprecipitation.  Smart-IP improves efficiency by reducing time-consuming steps such as centrifugation.  The magnetic beads and magnetic agarose beads can be easily isolated using a magnetic rack. The supernatant can also be extracted with minimal sample loss resulting in high yields of your target position.

  • High protein recovery
  • No pre-clear step
  • Complete removal of supernatant
  • Ready to use        

Smart-IP1ver2-768x336

Smart-IP2_2-1024x409

Description of Each Conjugate

  Magnetic Beads MAGNETIC AGAROSE AGAROSE
Applications
 IP
 IP, Purification
 IP, Purification
Bead Diameter
 1.5 μm
 50 μm 
 100 μm
 Schematic Diagram
IP1 IP3
IP2
 Product Images
smart1
 smart2
  smart3
Pros
Almost no sample lossEasy to see the beadsEasy to disperse, can be used for screening
Large amount of IgG/per beadsLess sample lossEasy to see the beads
Large amount of IgG/per beadsNo need of magnetic rackLower cost
Cons
Small amount of IgG/per beadsMagnetic rack will be needed
 Magnetic rack will be needed
Might lose sample during the washHard to see the gel

Product List by Epitope Tag

EPITOPE TAG Clonality CLONE NUMBER ISOTYPE SIZES CODE NO.: CONJUGATE TO MAGNETIC BEADS CODE NO.: CONJUGATED TO MAGNETIC AGAROSE CODE NO.: CONJUGATED TO AGARAOSE
DDDDK
Monoclonal
FLA-1
Mouse IgG2ak
1 mL
GFP
Monoclonal
RQ2
Rat IgG2ak
1 mL
HA
Monoclonal
5D8
Mouse IgG1k
1 mL
HA
Monoclonal
TANA2
Mouse IgG2bk
1 mL
 
His
Monoclonal
OGHis 
Mouse IgG2ak
1 mL
Myc
Monoclonal
PL14
Mouse IgG1k
1 mL
RFP
Monoclonal
3G5
Mouse IgG1k
1 mL
 
V5
Monoclonal
1H6
Mouse IgG2ak
1 mL 

 

Trial sizes available, contact us for details.

 

References:

  1. Cai, L., et al., J. Biol. Chem. 286, 35915-35921 (2011)
  2. Sato, Y., et al., J. Biol. Chem. 284, 11873-11881 (2009)
  3. Sakurai, T., et al., J. Cell Biol. 183, 339-352 (2008)