Smart IP
Smart IP: Fast and Effective IP
Smart-IP is a line of highly specific tag antibodies conjugated to magnetic beads or magnetic agarose beads designed for faster immunoprecipitation. Smart-IP improves efficiency by reducing time-consuming steps such as centrifugation. The magnetic beads and magnetic agarose beads can be easily isolated using a magnetic rack. The supernatant can also be extracted with minimal sample loss resulting in high yields of your target position.
- High protein recovery
- No pre-clear step
- Complete removal of supernatant
- Ready to use
Description of Each Conjugate
| Magnetic Beads | Magnetic Agarose | Agarose | |
|---|---|---|---|
| Applications | IP | IP, Purification | IP, Purification |
| Bead Diameter | 1.5 μm | 50 μm | 100 μm |
| Schematic Diagram |  |  |  |
| Product Images |  |  |  |
| Pros | Almost no sample lossEasy to see the beadsEasy to disperse, can be used for screening | Large amount of IgG/per beadsLess sample lossEasy to see the beads | Large amount of IgG/per beadsNo need of magnetic rackLower cost |
| Cons | Small amount of IgG/per beadsMagnetic rack will be needed | Magnetic rack will be needed | Might lose sample during the washHard to see the gel |
Product List by Epitope Tag
| Epitope Tag | Clonality | Clone Number | Isotype | Sizes | Code No.: Conjugate to Magnetic Beads | Code No.: Conjugated to Magnetic Agarose | Code No.: Conjugated to Agaraose |
|---|---|---|---|---|---|---|---|
| DDDDK | Monoclonal | FLA-1 | Mouse IgG2ak | 1 mL | M185-11 | M185-10 | 3325 |
| GFP | Monoclonal | RQ2 | Rat IgG2ak | 1 mL | D153-11 | D153-10 | D153-8 |
| HA | Monoclonal | 5D8 | Mouse IgG1k | 1 mL | M132-11 | M132-10 | 3320 |
| HA | Monoclonal | TANA2 | Mouse IgG2bk | 1 mL | M180-11 | M180-10 | |
| His | Monoclonal | OGHis | Mouse IgG2ak | 1 mL | D291-11 | D291-10 | D291-8 |
| Myc | Monoclonal | PL14 | Mouse IgG1k | 1 mL | M047-11 | M047-10 | M047-8 |
| RFP | Monoclonal | 3G5 | Mouse IgG1k | 1 mL | M165-11 | M165-8 | |
| V5 | Monoclonal | 1H6 | Mouse IgG2ak | 1 mL | M167-11 | M167-10 | 3315 |
Trial sizes available, contact us for details.
References:
- Cai, L., et al., J. Biol. Chem. 286, 35915-35921 (2011)
- Sato, Y., et al., J. Biol. Chem. 284, 11873-11881 (2009)
- Sakurai, T., et al., J. Cell Biol. 183, 339-352 (2008)
