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MBL International provides high-quality products and solutions to advance life science research and clinical diagnostics.

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Product Highlight

MHC Tetramers

 

MHC Tetramers and Monomers for high specificity immune monitoring.

Patented α3 mutation for reduced background.

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Product Highlight

TransFix®: A Cellular Antigen Stabilization Reagent

 

TransFix® is a versatile cellular antigen stabilization solution that prevents cellular degradation in a variety of specimen types for flow cytometric analysis.

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Product Highlight

p62 ELISA Kits

 

MBL International introduces a line of p62 ELISA Kits that complement our p62 antibodies and extensive autophagy antibody portfolio.

p62 ELISA Kits are useful as a marker for autophagy.

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Product Highlight

Autoimmune ANA Test Systems

We offer screens for a wide range of autoantibodies with each lot tested to ensure lot to lot sensitivity.

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TransFix/EDTA Vacuum Blood Collection Tubes (TVTs)

TransFix Vacuum Tubes are now available for in vitro diagnostic (IVD) use in the USA, for immunophenotyping of white blood cells by flow cytometry.

By stabilizing samples with TransFix/EDTA Vacuum Blood Collection Tubes, recovery of lymphocyte subset markers for the HIV panel can be accomplished over a 14-day period following collection. This is particularly important in the routine diagnosis and immune monitoring of HIV patients.

Learn More about this TransFix product.

 

 

 

 

COLLABORATE WITH MBLI

Partnering with pharmaceutical, biotechnology, and diagnostics companies to out-license our innovative technologies and products supporting life science research, develop companion diagnostics and in-license IVD technologies/products in the areas of cancer, autoimmune diseases, infectious diseases, allergy and cytological/pathological testing.

  • Mechanisms and detection methods of p62/SQSTM1 and its importance in the autophagy pathway.

    Tue, 09 Jan 2018 15:24:11 GMTBy: nakajo.tomohiro@mbl.co.jp (Tomohiro Nakajo)

    Mechanisms of Autophagy
    The ubiquitin-conjugating protein p62/SQSTM1 is thought to be a scaffold protein that interacts with a variety of molecules involved in toll-like receptor (TLR) signaling, such as TRAF6, ERK, and aPKC. Recent evidence suggests that p62 binds directly to the autophagosome marker LC3 and is then selectively degraded by autophagy.
    p62 contains multiple phosphorylation sites. Sequential phosphorylation of these sites regulates biological defense mechanisms such as selective autophagy and the Keap1-Nrf2 system. Therefore, the failure of the p62 pathway is associated with various diseases such as cancer and many neurodegenerative diseases.
    To learn more about the mechanisms of p62, please refer to our whitepaper written by Drs. Masaaki Komatsu and Yoshinobu Ichimura, Niigata University. They are leading researchers in Autophagy,  especially p62 and Keap1-Nrf2 interaction. Dr. Komatsu was recognized as one of the most highly cited researchers in Autophagy in 2014 and 2015 by Clarivate Analytics.

    p62 Detection
    How do you detect p62 or phospho-p62 using antibodies? Western blotting? Immunohistochemistry?  Quantification of biomarkers is essential for medical and diagnostic application. Selective autophagy and Keap1-Nrf2 are already being discussed for their use as a diagnostic as well as other medical applications.
    MBL International has developed new sandwich ELISA kits that can semi-quantify total p62 and its phosphorylation level at Ser403 and Ser349. Phospho-p62 ELISA kits are the first kits to quantify these markers by ELISA in the world research market. It is a chance to get a new discovery in the Autophagy area!

    CY-7055: CycLex® Total p62 ELISA Kit

    CY-7057: CycLex® Phospho-p62 Ser403 ELISA Kit

    CY-7056:  CycLex® Phospho-p62 Ser349 ELISA Kit

    These kits can help you to prepare cell lysate easily including cell lysis buffers. Phosphatase inhibitor solution is also included in the phosho-p62 kits. They can be used for human and mouse samples.
    Total p62 and phospho-p62 levels in HeLa cells Read more

  • Evaluating the biological activity of a cancer vaccine with the HLA-A*02:01 QuickSwitch™ Quant platform

    Mon, 11 Dec 2017 16:33:12 GMTBy:

    Immuno-oncology is a growing field in which different strategies such as immune modulators, vaccines and adoptive cell therapies are applied for new immunotherapies. Cancer vaccine immunogenicity and stability are two important parameters in vaccine development. In addition, vaccine immunogenicity implies accurate enumeration of antigen specific T-cells. MHC tetramers can help monitor peptide vaccine immunogenicity and stability. Read more
  • The problem with Ultracentrifugation for exosome isolation

    Thu, 02 Nov 2017 16:00:00 GMTBy: DMagcase@jsrmicro.com (Dennie Magcase)

    Ultracentrifugation is regarded as the gold standard method for exosome isolation.  The ultracentrifugation process is time consuming and may introduce protein and nucleic acid contamination.  Pre-clearing samples by one or more low speed centrifugation steps will deplete the cells, platelets, and large apoptotic bodies.    Larger extracellular vesicles can be pelleted at forces between 10000-20000 x g.  Smaller extracellular vesicles can be pelleted at higher speeds, ranging from 100000-200000 x g. Depending on the protocol, it can take up to 24 hours to isolate exosomes by ultracentrifugation.  It is impossible to achieve absolute separation due to potential contamination of proteins and nucleic acids.
    Figure 1. General Ultracentrifugation Protocol
    ExoCap™ Streptavidin Kit – The faster, easier and customizable
    The ExoCap™ Streptavidin kit protocol is very easy and simple to follow.  Researchers can isolate and enrich for exosomes of interest by coupling biotinylated molecules to the magnetic Streptavidin beads.  There is no need for pre-treatment of samples because there is a direct incubation of sample with the Streptavidin magnetic beads.  The magnetic Streptavidin beads coupled with biotinylated antibodies will selectively capture exosomes of interest.  During the wash step the captured exosomes on the magnetic bead are retained, while any contaminants are removed, resulting in high purity exosomes that can be used for downstream analyses in as little as 4 hours.
    Figure 2. ExoCap™ Streptavidin Kit Protocol
    ExoCap™ Streptavidin Kit vs Ultracentrifugation
    Table 1. Streptavidin Kit and Ultracentrifugation Comparison Read more

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  • p62 ELISA Kits– Complementary products to our p62 antibodies and extensive autophagy antibody portfolio
  • Stock and Custom Peptides – High-quality peptides for your desired specification.
  • QuickSwitch™ Custom Tetramer Kits – Create new specificity tetramers for antigen-specific t cell detection in just a few hours
  • TransFix® – A patented stabilization solution that is easy to use and preserves cells and cellular antigens for up to 14 days prior to analysis
  • Exosome Storage Booster – Improve the recovery rate of exosomes from cell culture supernatant using ultracentrifugation