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science.
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MBL International provides high-quality products and solutions to advance life science research and clinical diagnostics.

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Product Highlight

MHC Tetramers

 

MHC Tetramers and Monomers for high specificity immune monitoring.

Patented α3 mutation for reduced background.

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Product Highlight

TransFix®: A Cellular Antigen Stabilization Reagent

 

TransFix® is a versatile cellular antigen stabilization solution that prevents cellular degradation in a variety of specimen types for flow cytometric analysis.

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Product Highlight

p62 ELISA Kits

 

MBL International introduces a line of p62 ELISA Kits that complement our p62 antibodies and extensive autophagy antibody portfolio.

p62 ELISA Kits are useful as a marker for autophagy.

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Product Highlight

Autoimmune ANA Test Systems

We offer screens for a wide range of autoantibodies with each lot tested to ensure lot to lot sensitivity.

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TransFix/EDTA Vacuum Blood Collection Tubes (TVTs)

TransFix Vacuum Tubes are now available for in vitro diagnostic (IVD) use in the USA, for immunophenotyping of white blood cells by flow cytometry.

By stabilizing samples with TransFix/EDTA Vacuum Blood Collection Tubes, recovery of lymphocyte subset markers for the HIV panel can be accomplished over a 14-day period following collection. This is particularly important in the routine diagnosis and immune monitoring of HIV patients.

Learn More about this TransFix product.

 

 

 

 

COLLABORATE WITH MBLI

Partnering with pharmaceutical, biotechnology, and diagnostics companies to out-license our innovative technologies and products supporting life science research, develop companion diagnostics and in-license IVD technologies/products in the areas of cancer, autoimmune diseases, infectious diseases, allergy and cytological/pathological testing.

  • The problem with Ultracentrifugation for exosome isolation

    Thu, 02 Nov 2017 16:00:00 GMTBy: DMagcase@jsrmicro.com (Dennie Magcase)

    Ultracentrifugation is regarded as the gold standard method for exosome isolation.  The ultracentrifugation process is time consuming and may introduce protein and nucleic acid contamination.  Pre-clearing samples by one or more low speed centrifugation steps will deplete the cells, platelets, and large apoptotic bodies.    Larger extracellular vesicles can be pelleted at forces between 10000-20000 x g.  Smaller extracellular vesicles can be pelleted at higher speeds, ranging from 100000-200000 x g. Depending on the protocol, it can take up to 24 hours to isolate exosomes by ultracentrifugation.  It is impossible to achieve absolute separation due to potential contamination of proteins and nucleic acids.
    Figure 1. General Ultracentrifugation Protocol
    ExoCap™ Streptavidin Kit – The faster, easier and customizable
    The ExoCap™ Streptavidin kit protocol is very easy and simple to follow.  Researchers can isolate and enrich for exosomes of interest by coupling biotinylated molecules to the magnetic Streptavidin beads.  There is no need for pre-treatment of samples because there is a direct incubation of sample with the Streptavidin magnetic beads.  The magnetic Streptavidin beads coupled with biotinylated antibodies will selectively capture exosomes of interest.  During the wash step the captured exosomes on the magnetic bead are retained, while any contaminants are removed, resulting in high purity exosomes that can be used for downstream analyses in as little as 4 hours.
    Figure 2. ExoCap™ Streptavidin Kit Protocol
    ExoCap™ Streptavidin Kit vs Ultracentrifugation
    Table 1. Streptavidin Kit and Ultracentrifugation Comparison Read more
  • Protein analysis in circadian rhythm research

    Wed, 27 Sep 2017 16:00:00 GMTBy: nakajo.tomohiro@mbl.co.jp (Tomohiro Nakajo)

    Living organisms on the earth synchronize their activity to a 24 hour light and dark cycle generated by the rotation of the earth. This biological rhythm is called the circadian rhythm.
    Recently the molecular mechanism for the oscillation of the circadian rhythm has been elucidated and the approximately 24-hour-rhythm was found to be generated by a transcription-translation feedback-loop of clock genes expressed by almost all cells. In particular, BMAL1, CLOCK, PERs and CRYs play key roles in oscillation of the circadian rhythm and rhythmically regulate the expression of downstream genes (hereinafter referred to as clock-controlled genes [CCGs]).
    So far, the expression analysis of CCGs is mainly performed by mRNA quantification, like qRT-PCR and in situ hybridization. However, it is necessary to analyze protein expression level, post-translational modifications like phosphorylation, and ubiquitination for understanding the molecular mechanism of circadian rhythm. Actually, phosphorylation and ubiquitination of PER and ubiquitination of CRY are well known.
    Why are these protein expression analyses not well done? The answer is very simple: because there weren’t any good antibodies in this market, so we developed antibodies against CCGs.
    At first we developed antibodies against PER1/2, CRY1/2, BMAL1 and CLOCK, playing core feedback loop in circadian rhythm. BMAL1 and CLOCK form a heterodimer, which binds to the regulatory region (E-box) in per1/2 and cry1/2 to positively regulate transcription. PER and CRY proteins then form a complex, migrate to the nucleus, and negatively regulate the function of the BMAL1/CLOCK complex. CCGs with E-box elements are expressed and are regulated by the BMAL1/CLOCK complex, and various genes are expressed according to circadian rhythm.
    We successfully developed antibodies against all of the core feedback loop members. Anti-PER1, PER2, BMAL1 and CLOCK can be used in Immunohistochemistry (IHC), and anti-BMAL1 and CLOCK can be used for Chromatin Immunoprecipitation (ChIP) assays as well. Please be careful to choose secondary antibodies because some of the products are derived from guinea pig!
    Anti-PER2 (Mouse) pAb (Code# PM083) –Rabbit Ig (Aff.)
    Anti-PER1(Mouse) pAb (Code# PM091) – Guinea Pig Ig (Aff.) Read more
  • How to detect functional PCSK9 quickly and easily

    Tue, 27 Jun 2017 14:00:00 GMTBy: bindi.doshi@mblintl.com (Bindi M. Doshi, PhD)

    PCSK9 remains an important target in cholesterol research.  One of the hurdles in this research area is determining which PSCK9 form is present and binding with LDLR.  Our new Human PCSK9 Functional Assay Kit is designed to detect active PSCK9 binding with LDLR.  Read more

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  • p62 ELISA Kits– Complementary products to our p62 antibodies and extensive autophagy antibody portfolio
  • Stock and Custom Peptides – High-quality peptides for your desired specification.
  • QuickSwitch™ Custom Tetramer Kits – Create new specificity tetramers for antigen-specific t cell detection in just a few hours
  • TransFix® – A patented stabilization solution that is easy to use and preserves cells and cellular antigens for up to 14 days prior to analysis
  • Exosome Storage Booster – Improve the recovery rate of exosomes from cell culture supernatant using ultracentrifugation