QuickSwitch™ Custom Tetramer Kits

Fast, High-Quality Generation of New Tetramer Specificities

Functional screening of peptides for MHC class I binding is essential for vaccine design and immune monitoring.  A rapid, high throughput and user friendly assay system that has the potential for clinical immune monitoring is needed now more than ever.

We present a 90-min-assay system that allows discrimination of MHC binding from non-binding peptides.  This is particularly essential for the screening of immunogenic peptides from infectious agents or cancer neoantigens.  Tetramers resulting from peptide exchange with selected peptides can then be used for immune monitoring.

Browse our QuickSwitch™ products

Product DescriptionAlleleConjugates AvailableQuantification Included
QuickSwitch™ Quant HLA-A*02:01 Tetramer KitHLA-A*02:01PEAPCBV421Yes
QuickSwitch™ Quant HLA-A*03:01 Tetramer KitHLA-A*03:01PE, APC, BV421Yes
QuickSwitch™ Quant HLA-A*11:01 Tetramer KitHLA-A*11:01PEAPCBV421Yes
QuickSwitch™ Quant HLA-A*24:02 Tetramer KitHLA-A*24:02PE, APCBV421Yes
QuickSwitch™ Quant H-2 Kb Tetramer KitH-2KbPEAPCBV421Yes
QuickSwitch™ HLA-A*02:01 Peptide Screening KitHLA-A*02:01Yes

High Throughput Immunogenic Peptide Discovery and Validation in 90 min

QuickSwitch™ Platform Capabilities:

  • Validate MHC binding peptides from in silico selected list of candidate peptides
  • Generate new specificity tetramers for immune monitoring
  • Perform functional stability studies for MHC binding peptides
  • Compare epitopes to rank better binders and perform epitope mapping                             

Peptide exchange, quantification, cell staining, and flow cytometry analysis can all be performed in one day!

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QuickSwitch™ HLA-A*02:01 tetramers detect similar percentages of low and high affinity CMV responses in PBMCs as classically folded tetramers

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QuickSwitch™ Quant Kit can be used to assess peptide exchange so that you can select peptides with appropriate affinities to make functional tetramers prior to cell staining. In a study where HLA-A*02:01 QuickSwitch™ tetramer was incubated for 4 hours with two Mart-1 related peptides at a final of 20 μM in presence of peptide exchange factor #1, peptide exchange correlated with the theoretical peptide affinity of each peptide towards HLA-A*02:01.

QuickSwitchTM Quant kit components:

  • Tetramer with an irrelevant exchangeable peptide in the MHC groove
  • A peptide exchange factor for catalyzing the peptide exchange reaction
  • A high affinity MHC-binding reference peptide used as peptide exchange positive control
  • FITC conjugated antibody specific of the exiting peptide
  • Magnetic beads conjugated with anti MHC antibody for tetramer capture
  • An Assay Buffer for diluting reagents and washing steps

Webinar – Immune Monitoring with MHC Tetramers and QuickSwitch™ Rapid Tetramer Technology

H2-Kb Peptide-exchanged tetramers perform similarly to classically folded tetramers

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H-2 Kb TRP2 used a negative control (#TB-5004-2; green), classically folded H-2 Kb OVA (#TB-5001-2; blue), and H-2 Kb QuickSwitch™ OVA (red) tetramer staining.

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QuickSwitch™ HLA-A*24:02 tetramers perform similarly to classically folded tetramers

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Data show CD3+ PBMCs stained with classically folded tetramers (A,E) or with QuickSwitch™ tetramers obtained by peptide exchange with the HLA-A*24:02 CMV peptide (B,C,D) or the HIV negative control peptide (F,G,H). 2 x 105 cells in 50 μL PBS-BSA-NaN3 buffer were stained for 30 min at RT with 1 μL of anti CD3-PC5.5 mAb (clone OKT3), 1 μL anti CD8-FITC (clone RPA-T8) (MBL) and 0.25 μg tetramer. Cells were fixed with a 0.5% formaldehyde PBS solution. Cells were analyzed on a Cytoflex S flow cytometer (Beckman Coulter). Cell doublets were discriminated using SSC-W/SSC-A gating.

Learn more about our Related Product Lines

Classical MHC Tetramers and Monomers

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Stock and Custom Peptides

  • Stimulation Assays
  • Custom tetramer generation using QuickSwitch™ and QuickSwitch™ Quant Tetramer Kits
  • Stock peptides or designated custom sequences available

Flow Cytometry Antibodies and Reagents

  • Over 1,200 Products
  • Major targets such as CD4 and CD8
  • Popular fluorophores available
  • Antibodies, buffers, viability dyes, and more!

References

  1. Kula, T., Dezfulian, M. H., Wang, C. I., Abdelfattah, N. S., Hartman, Z. C., Wucherpfennig, K. W., … Elledge, S. J. (2019). T-Scan: A Genome-wide Method for the Systematic Discovery of T Cell Epitopes. Cell, 178(4).Available at:  https://www.ncbi.nlm.nih.gov/pubmed/31398327
  2. Christof C. Smith, Shengjie Chai, Amber R. Washington, Samuel J. Lee, Elisa Landoni, Kevin Field, Jason Garness, Lisa M. Bixby, Sara R. Selitsky, Joel S. Parker, Barbara Savoldo, Jonathan S. Serody, and Benjamin G. Vincent (2019) Machine-Learning Prediction of Tumor Antigen Immunogenicity in the Selection of Therapeutic Epitopes. Available at:  https://cancerimmunolres.aacrjournals.org/content/early/2019/09/11/2326-6066.CIR-19-0155