LC3 Antibodies

A yeast autophagy-related gene product (Atg8) has three mammalian homologues: LC3, GABAA receptor associated protein (GABARAP), and Golgi-associated ATPase enhancer (GATE-16).  Among them, LC3 is most actively studied and frequently used as a mammalian autophagy marker. Shortly after translation (proLC3), LC3 antibodies are processed at the C-terminus by Atg4B or Atg4A into LC3-I. Upon induction of autophagy, LC3-I is conjugated to the substrate phosphatidylethanolamine (PE) via E1 (Atg7) and E2 (Atg3). The PE-LC3-I conjugate is referred to as LC3-II. Despite having a higher molecular weight than LC3-I, LC3-II is more hydrophobic and shows a higher mobility in sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (SDS-PAGE). GABARAP and GATE-16 are also demonstrated to undergo the same process of conjugation with PE.

LC3 antibodies
LC3 AntibodyLC3 IsoformApplication
MAP1LC3AMAP1LC3BMAP1LC3CWBFCMIPPIHCICC
M152XXXXXXX
M186XX
PM036XXXXXXXX
PD014XXXX

LC3 has been found to have multiple isoforms. To date, we know of LC3A, LC3B, and LC3C. MBL International offers high quality LC3 antibodies specific for detecting exactly the isoform of interest. Our LC3 antibodies can be used for various applications including: western blots, immunohistochemistry, flow cytometry and more.

Top anti-LC3 Antibodies:

Code No.Product NameSpecies ReactivityApplications
M152-3Anti-LC3 (Human) Monoclonal AntibodyHuman, Mouse, Rat, HamsterFCM, ICC, IP, WB
M186-3Anti-LC3 (Human) Monoclonal AntibodyHuman, Mouse, Rat, HamsterWB
M186-7Anti-LC3 Monoclonal Antibody-HRP-DirecTHuman, Mouse, Rat, HamsterWB
PD014Anti-LC3 (Rat) Polyclonal AntibodyHuman, Mouse, Rat, HamsterWB
PM036Anti-LC3 (Human) Polyclonal AntibodyHuman, Mouse, Rat, HamsterFCM, ICC, IHC, IP, WB

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