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Epitope Tag Purification Kits and Gels

Purification Kit

This kit is optimized to purify target epitope-tagged proteins without any loss of protein activity or denaturation.

MBLI’s tagged Protein PURIFICATION KIT employs competitive polypeptides to elute target tagged proteins under a neutral pH to maintain target protein function and conformation. Epitope tag specific antibodies in this kit are highly optimized for target recovery with high yield and purity from crude samples like cell lysates or culture supernatant of mammalian transfectant cells. 

EpitopeTagPur1

This product is a set of purification gel, elution peptide, wash buffer, and mini column

Procedure Summary

EpitopePur2

Tagged Protein Magnetic Purification Kits

Purification1_1

  • Contains magnet-conjugated antibodies, elution peptide, and wash solutions
  • Recommended for purifying small amounts of sample 
  • No need for centrifugation
  • High yield
  • Maintain target protein function and conformation

Procedure Summary

EpitopePur15-1024x304

c-Myc-tagged GST protein purification (Product Code # 3340)

EpitopePur16

V5-tagged β-galactosidase purification (Product Code # 3341)

EpitopePur17

Purified in native conformations

Maintain target protein function and conformation under a neutral pH condition using MBLI’s purification procedure. This procedure is useful for pull down assay as well as analysis of co-immunoprecipitated proteins.

Purification and enzymatic activity of N-terminal His-tagged β-galactosidase (Product Code # 3310)

EpitopePur3

Pull-down assay (Product Code # 3320)

EpitopePur4

High Purity

Recover high-purity tagged proteins by using our original proprietary antibodies that have superior affinity and specificity to the epitope tag.

Purification of C-terminal His-tagged protein X from culture supernatant.

EpitopePur5

PURIFICATION GEL with Elution Peptide

EpitopePur7

Recover high-purity tagged proteins by using our original proprietary antibodies that have superior affinity and specificity to the epitope tag.

Purification of C-terminal His-tagged protein X from culture supernatant (Product Code # 3311)

EpitopePur8_1-1024x439

Purification Gel

EpitopePur9

This product consists of a purification gel only.

Purification of N-terminal DDDDK-tagged β-galactosidase (Product Code # 3328)

EpitopePur10-1024x285

Purification Cartridge 

EpitopePur11

*Ready-to-use-cartridge for the isolation of c-Myc-tagged
*The cartridge can be used directly on automated AKTA or FPLC systems. (Cartridge is 1/16 inch female end)

Purification cartridge (Product Code # 3306K)

EpitopePur13_1-1024x474