RiboCluster Profiler™ RIP-Assay™
The RiboCluster Profiler™ RIP-Assay Kit utilizes a technique named RIP-Chip (ribonucleoprotein immunoprecipitation-microarray profiling) which is a biochemical approach to identify the composition and organization of endogenous mRNAs, miRNAs and RNA binding proteins (RBPs) within messenger ribonucleoprotein (mRNP) complexes.
When the co-isolated miRNA and mRNA subpopulations are analyzed using the computational predictions of conserved seed sequences, this approach provides a powerful tool to identify functional miRNA targets based on their physical interaction in vivo. RBPs have been reported to bind to mRNAs that encode functionally related proteins and coordinate regulation of these mRNAs during cellular processes. The RIP-Chip approach can isolate functionally related mRNAs. Since miRNAs can be co-immunoprecipitated with those mRNAs, the RIP-Chip approach can isolate miRNAs that regulate specific group of mRNAs that are functionally related.