What are antibodies?
Antibodies are proteins produced and secreted by B cells. They bind to foreign substances that invade the body, such as pathogens. The term "antibody" refers to its function, which is to bind to an antigen. Another name for this protein molecule is immunoglobulin (abbreviated Ig).
Antibodies are Y-shaped molecules, consisting of two heavy chains (H chains) and two light chains (L chains) arranged as shown in the diagram on the right. An antigen binds to the antigen-binding site at the tip of the "Y." An important feature is that each antibody recognizes a specific antigen as illustrated in the diagram. This is called "antibody specificity". (Details are described in The role of antibodies).
Next page: Structure of antibodies
→Antibodies as a research tool
- How to generate antibodies
- How to select antibodies
- Labeled antibodies
- How to label antibodies
- Main causes of non-specific reactions
- How to reduce non-specific reactions
- Tags and Tag antibodies
*Qualitative and quantitative measurements of proteins using antibodies
- Western blotting (WB)
- Enzyme-linked Immunosorbent assay (ELISA)
- Immunoprecipitation (IP)
- Co-immunoprecipitation (Co-IP)
*Fractionation and purification of proteins