TransFix®: A Cellular Antigen Stabilization Reagent

Patient samples, such as whole blood, cerebrospinal fluid (CSF), circulating tumor cells, and bone marrow, are often analyzed for their immunophenotypic profile after collection for various assays. However, cell surface antigens start degrading immediately upon venipuncture and lumbar puncture, particularly leukocytes within CSF samples1. Often times, these precious cells break down before appropriate analysis can be done on the sample, resulting in repeat patient sample drawings. To avoid painful and costly repeat drawings, cells can be stabilized using TransFix until appropriate testing can be completed. TransFix is a patented stabilization solution that is easy to use and preserves cells and cellular antigens for up to 14 days prior to analysis2                                                                                                                                                                                         

Transfix

 Why Use TransFix:

*Stabilizes cell surface antigens and prevents cellular degradation for up to 14 days
*Preserves sample integrity during shipment between sites
*Permits sample batching
*Enables repeat testing without requiring patient recall

Transfix has been used to stabilize:

*Human whole blood (up to 14 days)
*Cerebrospinal fluid (up to 72 hours)
*Bone Marrow
*Circulating Tumor Cells
*Lymph Node Biopsies
*Animal Blood

 

Sample Transport & Batching

The use of TransFix is beneficial in situations where sample storage, batching or transportation is required prior to analysis. TransFix stabilized samples can be stored and transported at ambient temperature. Since samples can be stored as a batch, the need for frequent deliveries can be reduced. TransFix also reduces the need to recall patients for additional phlebotomy by extending the window of opportunity for sample testing, resulting in significant cost savings3.

Easy to Use

Samples are treated with TransFix as soon as possible after phlebotomy. Samples are added to TransFix, inverted and then stored. The stabilized sample can now be stored until it is convenient to analyze.

                                                                                                       TF1

Circulating Tumor Cell TransFix/EDTA Vacuum Blood Collection Tubes - RUO

TransfiThe number of circulating tumor cell populations present in blood is very low, therefore the usefulness of CTC assessments depends upon accurate cell counts and the corresponding analysis of molecular targets. Circulating Tumor Cell TransFix/EDTA Vacuum Blood Collection Tubes are 9ml blood collection tubes prefilled with an optimised volume of TransFix for the collection and stabilisation of circulating tumour cells in whole human blood. They allow stabilisation of CTCs for up to 5 days at ambient temperature (18-25°C), immediately preventing the degradation of CTCs and CTC antigens, allowing time to isolate and analyze these rare cells though multiple methodologies.

View Poster Here

Code # Name Size
CTC-TVT-09-2 Circulating Tumor Cell TransFix/EDTA Vacuum Blood Collection Tubes 2 x 9 mL tube
CTC-TVT-09-50 Circulating Tumor Cell TransFix/EDTA Vacuum Blood Collection Tubes 50 x 9 mL tube

References 

1) de Graaf, M. T., de Jongste, A. H. C., Kraan, J., Boonstra, J. G., Smitt, P. A. E. S. and Gratama, J. W. (2011), Flow cytometric characterization of cerebrospinal fluid cells. Cytometry, 80B: 271–281. doi:10.1002/cyto.b.20603

2) Canonico, B., Betti, M., Luchetti, F., Battistelli, M., Falcieri, E., Ferri, P., Zamai, L., Barnett, D. and Papa, S. (2010), Flow cytometric profiles, biomolecular and morphological aspects of transfixed leukocytes and red cells. Cytometry, 78B: 267–278. doi:10.1002/cyto.b.20510 

3) Vicky Y. Hoymansa, Amaryllis H. Van Craenenbroecka, Luc Bruyndonckxa, , Sabrina H. van Ierssela, Christiaan J. Vrintsa, Viviane M. Conraadsa, Emeline M. Van Craenenbroecka, “TransFix® for Delayed Flow Cytometry of Endothelial Progenitor Cells and Angiogenic T Cells.” Microvascular Research, Volume 84, Issue 3, November 2012, Pages 384–386.

Additional References:

BLOOD (RESEARCH)

1) N. Poirier, G. Blancho, M. Hiance, C. Mary, T. Van Assche, J. Lempoels, S. Ramael, W. Wang, V. Thepenier, C. Braudeau, N. Salabert, R. Josien, I. Anderson, I. Gourley, J. Soulillou, D. Coquoz, B. Vanhove. “First-in-Human Study in Healthy Subjects with FR104, a Pegylated Monoclonal Antibody Fragment Antagonist of CD28” Journal of Immunology. DOI: https://doi.org/10.4049/jimmunol.1601538

2) Branwen Hennig , Digna Velez-Edwards, Maarten Schim van der Loeff, Cyrille Bisseye, Todd Edwards, Alessandra Tacconelli, Giuseppe Novelli, Peter Aaby, Steve Kaye, William Scott, Assan Jaye, Hilton Whittle, Scott Williams, Adrian Hill, Giorgio Sirugo. “CD4 Intragenic SNPs Associate With HIV-2 Plasma Viral Load and CD4 Count in a Community-Based Study From Guinea-Bissau, West Africa” Journal of Acquired Immune Deficiency Syndromes (2011) 56, 1-8

3) E. Konsta, E. Kwvota “Method validation and immunophenotypical study of NK and T lymphocytes by flow cytometry” National and Kapodistrian University of Athens. http://phdtheses.ekt.gr/eadd/handle/10442/26992  Baum L, Crowe S. Landay AL “Advances in CD4 cell enumeration in resource-poor countries” Current Opinion in HIV and AIDS (2007) 2, 157-245

CEREBROSPINAL FLUID

1) Campos, L. Trujillo, D. López, L. Beltrán, E. Arias, G. Vélez, A. Infante, I. De Los Reyes, M. Vizcaíno, P. C. Guzmán, M. V. Herrera, J. Solano, D. Londo?o, A. Ca?as, F. Pretelt, J. C. Pérez, C. Cardozo, S. Fiorentino, S. Quijano “Study of body fluid samples using flow cytometry: Six years of experience at the Hospital Universitario San Ignacio -Ponti cia Universidad Javeriana, Bogota – Colombia” Universitas Scientiarum, 22(2): 123 - 143, 2017. doi: 10.11144/Javeriana.SC22-2.sobf

2) Ulrika Johansson, David Bloxham, Stephen Couzens, Jennifer Jesson, Ricardo Morilla, Wendy Erber, Marion Macey and British Committee for Standards in Haematology “Guidelines on the use of multicolor flow cytometry in the diagnosis of haematological neoplasms” British Journal of Haematology, Volume 165, Issue 4, pages 455–488, May 2014

3) H. de Jongste, J. Kraan, P. D. van den Broek, R. A. Brooimans, J. E. Bromberg, K. A. van Montfort, P. A. Sillevis Smitt, J. W. Gratama “Use of TransFix cerebrospinal fluid storage tubes prevents cellular loss and enhances flow cytometric detection of malignant hematological cells after 18 hours of storage” Cytometry Part B: Clinical Cytometry Volume 86, Issue 4, pages 272–279, July 2014

4) U.Johansson, M. Crawford, M. Hughes, K. Day, D. Harrison, T. Almond “Infiltration of CNS by acute leukaemia: Analysis of fresh and TransFix stabilised CSF. View Poster

5)  Tim Almond, Daniel Harrison, Mark Hughes, Michelle Crawford, Ulrika Johansson “Validating the use of TransFix to stabilize cerebrospinal fluid (CSF) for flow cytometry immunophenotyping of haematological malignancies” View Poster

BONE MARROW 

1) Sanchez-Munoz et al (2011). Immunophenotypic Characterisation of Bone Marrow Mast Cells in Mastocytosis and other Mast Cell Disorders. Methods in Cell Biology (103): 333-359

2) Beiral et al (2014). The Impact of Stem Cells on Electron Fluxes, Proton Translocation, and ATP Synthesis in Kidney Mitochondria After Ischemia/Reperfusion. Cell Transplantation (23): 207-220

CIRCULATING TUMOR CELLS (CTC)

1) Magbanua MJM, Pugia M, Lee JS, Jabon M, Wang V, Gubens M, et al. “A Novel Strategy for Detection and Enumeration of Circulating Rare Cell Populations in Metastatic Cancer Patients Using Automated Microfluidic Filtration and Multiplex Immunoassay.” (2015) PLoS ONE 10(10): e0141166. doi:10.1371/journal.pone.0141166

2) P. A. Fasching et al., “4EVER: Assessment of circulating tumor cells with a novel, filtration-based method, in a phase IIIb multicenter study for postmenopausal, HER2- negative, estrogen receptor-positive, advanced breast cancer patients.” J Clin Oncol 31, 2013 (suppl; abstr 591) View Poster Here

3) Z. Mu, N. Benali-Furet, G. Uzan, A. Znaty, Z. Ye, C. Paolillo, C. Wang, L. Austin, G. Rossi, P. Fortina, H. Yang, M. Cristofanilli “Detection and Characterization of Circulating Tumor Associated Cells in Metastatic Breast Cancer” Int. J. Mol. Sci (2016) 17(10): 1665

Animal Blood

1) C.Seliger, B. Schaerer, M. Kohn, H. Pendl, S. Weigend, B.Kaspers, S. Härtle “A rapid high-precision flow cytometry based technique for total white blood cell counting in chickens” Veterinary Immunology and Immunopathology, Volume 145, pages 86–99, 2012

2) Dennis Rubbenstroth, Tina S. Dalgaard, Sonja Kothlow, Helle R. Juul-Madsen and Silke Rautenschlein. “Effects of Cyclosporin A induced T-lymphocyte depletion on the course of avian Metapneumovirus (aMPV) infection in turkeys” Developmental & Comparative Immunology (2010) 34, Issue 5, pages 518-529

Other Sample Types

1) Karsten, E. , Sung, J. , Morgan, C. , Herbert, B. and Vesey, G. (2014) “Evaluation of TransFix® Mediated Stabilisation of Adipose-Derived Stromal Vascular Fraction for Delayed Flow Cytometry Analysis.” Open Journal of Regenerative Medicine, 3, 54-63. doi: 10.4236/ojrm.2014.33007.

2) A Noel, K Maghni, C Dion, KJ Wilkinson, S. Hallé, R. Tardif, G. Truchon. “Effects of nano-TiO 2 aerosols showing two distinct agglomeration states on rat lungs” Toxicology Letters, Volume 214, Issue 2, 17 October 2012, Pages 109–119

*Cytomark has not verified these findings and we recommend that internal validation of the product must be completed by the end user before considering use of the products in a clinical setting.

 

See Products