TransFix®/EDTA Vacuum Blood Collection Tubes
Patient blood samples are often analyzed for their immunophenotypic profile after collection for various assays. However, cells and other surface antigens start degrading immediately upon venipuncture. Without preservation, these precious cells break down before appropriate analysis can be done on the sample, resulting in repeat patient sample drawings. To avoid inconvenience to the patient and costly repeat drawings, cells can be stabilized using TransFix/EDTA Vacuum Blood Collection tubes (TVTs) until appropriate testing can be completed. The TVTs contain a patented stabilization solution, TransFix, which preserves cells and cellular antigens for up to 14 days prior to analysis1.
Why Use TransFix TVTs:
*Stabilizes cell surface antigens and prevents cellular degradation for up to 14 days
*Preserves sample integrity during shipment between sites
*Permits sample batching
*Enables repeat testing without requiring patient recall
TransFix Vacuum Blood Collection Tubes are for use in diagnostic procedures
TransFix/EDTA Vacuum Blood Collection Tubes (TVTs) are intended for collection and storage of human whole blood specimens for immunophenotyping of white blood cells by flow cytometry. Recovery of lymphocyte subset marker of the HIV panel can be accomplished over a 14-day period following collection. (CD3, CD8, CD4, CD45, CD16/CD56, CD19).
Sample Transport & Batching - Benefits of TVTs for Clinical Research
The use of TransFix TVTs is beneficial in situations where sample storage, batching or transportation is required prior to analysis. TransFix stabilized samples can be stored and transported at ambient temperature. Since samples can be stored as a batch, the need for frequent deliveries can be reduced. TVTs also reduce the need to recall patients for additional phlebotomy by extending the window of opportunity for sample testing, resulting in significant cost savings2.
Many clinical trials will collect samples from different sites. TransFix allows samples to be preserved at the same time point, and shipped to a single testing facility, reducing variability3. This has particular benefit when measuring the immune profile of subjects at frequent time points post drug administration4.
Easy to Use
Samples are collected in TVTs during phlebotomy. They contain the correct amount of TransFix/EDTA to stabilize the volume of blood for which the tube is designed to fill. Just mix by inversion and the stabilized sample is now ready for analysis.
|TVT-03-2-US||TransFix/EDTA Vacuum Blood Collection Tubes||2 x 3 mL|
|TVT-03-50-US||TransFix/EDTA Vacuum Blood Collection Tubes||50 x 3 mL|
TransFix Reagent - GPR
TransFix reagent is used for preservation of cellular antigen expression in biological samples such as whole human blood, cerebral spinal fluid, bone marrow, lymph nodes, circulating tumor cells and other specimen types. TransFix reagent can be optimized for each sample type so it is stabilized with a custom amount of reagent, further minimizing cost. TransFix reagent is provided in bulk volumes, to provide customers with more flexibility for their application.
|TF-B1-1||1mL TransFix||1 mL|
|TF-B20-1||20mL TransFix||20 mL|
|TF-B1-10||10 x 1mL TransFix||10 x 1 mL|
|TF-B1-50||50 x 1mL TransFix||50 x 1 mL|
TransFix Sample Storage Tubes (1.2ml) - GPR
TransFix Sample Storage Tubes are ready to use collection tubes, including 0.2ml TransFix. A 1ml aliquot of a sample such as whole blood should be transferred to the TransFix Sample Storage Tube as soon as possible to minimize cellular degradation. Now the patient’s sample can be analyzed by flow cytometry at the clinician’s convenience up to 14 days later.
|TF-1-2||TransFix Sample Storage Tubes (2 x 1.2mL tubes)||2 x 1.2 mL tubes|
|TF-1-10||TransFix Sample Storage Tubes (10 x 1.2mL tubes)||10 x 1.2 mL tubes|
|TF-1-25||TransFix Sample Storage Tubes (25 x 1.2mL tubes)||25 x 1.2 mL tubes|
|TF-1-50||TransFix Sample Storage Tubes (50 x 1.2mL tubes)||50 x 1.2 mL tubes|
TransFix Sample Storage Tubes (5ml) - GPR
TransFix 5 ml Sample Storage Tubes are ready to use collection tubes, including 0.2ml TransFix. 5ml TransFix Sample Storage Tubes are suitable where a lower ratio of TransFix to sample is required, for example, for sample types with low cell numbers such as cerebral spinal fluid.
|TF-5-2||TransFix Sample Storage Tubes (2 x 5mL tubes, suitable for samples with low cell numbers)||2 x 5 mL tubes|
|TF-5-10||TransFix Sample Storage Tubes (10 x 5mL tubes, suitable for samples with low cell numbers)||10 x 5 mL tubes|
|TF-5-25||TransFix Sample Storage Tubes (25 x 5 mL tubes, suitable for samples with low cell numbers)||25 x 5 mL tubes|
|TF-5-50||TransFix Sample Storage Tubes (50 x 5 mL tubes, suitable for samples with low cell numbers)||50 x 5 mL tubes|
1) Canonico, B., Betti, M., Luchetti, F., Battistelli, M., Falcieri, E., Ferri, P., Zamai, L., Barnett, D. and Papa, S. (2010), Flow cytometric profiles, biomolecular and morphological aspects of transfixed leukocytes and red cells. Cytometry, 78B: 267–278. doi:10.1002/cyto.b.20510
2) Daniel Harrison, Rosalie Ward, Sarah Bastow, Andrew Parr, Susan Macro, Paul Wallace “Inter-laboratory Comparison of the TransFix/EDTA Vacuum Blood Collection Tube (TVT) with the 5 mL Cyto-Chex® BCT
3) Vicky Y. Hoymansa, Amaryllis H. Van Craenenbroecka, Luc Bruyndonckxa, , Sabrina H. van Ierssela, Christiaan J. Vrintsa, Viviane M. Conraadsa, Emeline M. Van Craenenbroecka, “TransFix® for Delayed Flow Cytometry of Endothelial Progenitor Cells and Angiogenic T Cells.” Microvascular Research, Volume 84, Issue 3, November 2012, Pages 384–386.
4) N. Poirier, G. Blancho, M. Hiance, C. Mary, T. Van Assche, J. Lempoels, S. Ramael, W. Wang, V. Thepenier, C. Braudeau, N. Salabert, R. Josien, I. Anderson, I. Gourley, J. Soulillou, D. Coquoz, B. Vanhove. “First-in-Human Study in Healthy Subjects with FR104, a Pegylated Monoclonal Antibody Fragment Antagonist of CD28” J Immunol DOI: https://doi.org/10.4049/jimmunol.1601538