HLA-A*02:01 – PE Tetramer for APB Kit

Description

For use with PhenomeX  Opto^® T Cell Profiling workflow.  Please see PhenomeX website for complete product instructions and use.

Recommended to purchase 3 vials of  TB-T07300-1-BL: HLA-A*02:01 – PE Tetramer for APB Kit for each Opto^® T Cell Profiling workflow.

Opto® APB KIT OVERVIEW

The Opto® APB kit addresses a critical bottleneck in the tumor antigen discovery process – immunogenicity validation. The kit can be used to measure binding of putative antigenic peptides to HLA Class I, measure stability of peptide-HLA complexes over time, and, in conjunction with costimulatory antibodies pre-conjugated to the APBs, efficiently stimulate and expand endogenous antigen-specific T cells. In this manner, the Opto® APBs can replace donor-derived antigen-presenting cells, removing the inherent variability and lack of quality control associated with cell-based antigen presentation. Please visit PhenomeX for more information.

ANTIGEN-SPECIFIC T CELL EXPANSION

The Opto® APB kit is also capable of rapidly expanding rare endogenous, antigen-specific T cells from healthy donor peripheral blood more efficiently than dendritic cells (DCs). After measuring the capacity of SLC45A2 peptide to bind and maintain a stable peptide-HLA complex, we expanded T cells reactive to SLC45A2 using either Opto® APBs or monocyte-derived dendritic cells. Antigen-specific T cells are detected using a fluorescently labeled tetramer, which can be purchased directly from MBL International.