HLA-A*02:01 – PE Tetramer for APB Kit
Validate immunogenicity with a high degree of quality control and avoid screening numerous peptides per protein in order to identify a few antigens.
For use with PhenomeX Opto® T Cell Profiling workflow. Please see PhenomeX website for complete product instructions and use.
Recommended to purchase 3 vials of TB-T07300-1-BL: HLA-A*02:01 – PE Tetramer for APB Kit for each Opto® T Cell Profiling workflow.
Opto® APB KIT OVERVIEW
The Opto® APB kit addresses a critical bottleneck in the tumor antigen discovery process – immunogenicity validation. The kit can be used to measure binding of putative antigenic peptides to HLA Class I, measure stability of peptide-HLA complexes over time, and, in conjunction with costimulatory antibodies pre-conjugated to the APBs, efficiently stimulate and expand endogenous antigen-specific T cells. In this manner, the Opto® APBs can replace donor-derived antigen-presenting cells, removing the inherent variability and lack of quality control associated with cell-based antigen presentation. Please visit PhenomeX for more information.
ANTIGEN-SPECIFIC T CELL EXPANSION
The Opto® APB kit is also capable of rapidly expanding rare endogenous, antigen-specific T cells from healthy donor peripheral blood more efficiently than dendritic cells (DCs). After measuring the capacity of SLC45A2 peptide to bind and maintain a stable peptide-HLA complex, we expanded T cells reactive to SLC45A2 using either Opto® APBs or monocyte-derived dendritic cells. Antigen-specific T cells are detected using a fluorescently labeled tetramer, which can be purchased directly from MBL International.
|Research Area / Disease:||Immune Monitoring, Immunology|
|Regulatory Statement:||For Research Use Only. Not for use in diagnostic procedures.|
1 vial (50 tests) PE Switchable Tetramer (HLA*02:01)