QuickSwitch™ Quant H-2 Kb Tetramer Kit-BV421

Specifications:

Background

This QuickSwitch Starter Guide shows how to use our QuickSwitch kits with clear illustrations and step by step directions.

Contact us with any specific questions you may have!

Components

  • Tetramer with an irrelevant exchangeable peptide in the MHC groove
  • A peptide exchange factor for catalyzing the peptide exchange reaction
  • A high affinity MHC-binding reference peptide used as peptide exchange positive control
  • FITC conjugated antibody specific of the exiting peptide
  • Magnetic beads conjugated with anti MHC antibody for tetramer capture
  • An Assay Buffer for diluting reagents and washing steps

Description

QuickSwitch™ Tetramer Kit utilizes a patented technique for exchanging up to ten peptides on an MHC class I tetramer. Reduce time and cost with this kit to create your own custom tetramers.  The resulting custom tetramers can be used for multiple applications, including screening immunogenic peptides.

Target: Custom
Product Type: Tetramer
Size: 25 µg
Application: FCM
Research Area / Disease: Immune Monitoring, Immunology
Class: Class I
Allele: H-2Kb
Peptide Sequence: Custom
Conjugate: BV421
Species Reactivity: Mouse
Specificity: Custom
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Citations

Marc C Delcommenne, Olga Hrytsenko, Cynthia Tram, Genevieve Weir and Marianne M. Stanford. (2017) The QuickSwitch Quant HLA-A*02:01 Tetramer Kit can be used for determining the biological activity of a cancer vaccine. J Immunol, 198 (1 Supplement) 79.27; https://www.jimmunol.org/content/198/1_Supplement/79.27

Kula, T., Dezfulian, M. H., Wang, C. I., Abdelfattah, N. S., Hartman, Z. C., Wucherpfennig, K. W., … Elledge, S. J. (2019). T-Scan: A Genome-wide Method for the Systematic Discovery of T Cell Epitopes. Cell, 178(4). DOI: 10.1016/j.cell.2019.07.009

Christof C. Smith, Shengjie Chai, Amber R. Washington, Samuel J. Lee, Elisa Landoni, Kevin Field, Jason Garness, Lisa M. Bixby, Sara R. Selitsky, Joel S. Parker, Barbara Savoldo, Jonathan S. Serody, and Benjamin G. Vincent (2019) Machine-Learning Prediction of Tumor Antigen Immunogenicity in the Selection of Therapeutic Epitopes dok: 10.1158/2326-6066.CIR-1900155

Song, X., Lu, Z., & Xu, J. (2020). Targeting cluster of differentiation 47 improves the efficacy of anti‑cytotoxic T‑lymphocyte associated protein 4 treatment via antigen presentation enhancement in pancreatic ductal adenocarcinoma. Experimental and Therapeutic Medicine. doi:10.3892/etm.2020.9054

Najafabadi, A. H., Zhang, J., Aikins, M. E., Abadi, Z. I., Liao, F., Qin, Y.,  Moon, J. J. (2020). Cancer Immunotherapy via Targeting Cancer Stem Cells Using Vaccine Nanodiscs. Nano Letters, 20(10), 7783-7792. doi:10.1021/acs.nanolett.0c03414

Son, E. T., Faridi, P., Paul-Heng, M., Leong, M., English, K., Ramarathinam, S. H., . . . Sharland, A. F. (2020). The self-peptide repertoire plays a critical role in transplant tolerance induction. Biorxiv. doi:10.1101/2020.11.09.359968

References

  1. Kubota, H., et al., J. Immunol. 145, 3924-3931 (1990)
  2. Hans et al, GWAS_Supplementary Information
  3. Asthma GWAS paper_Nat Comm
Code TB-7400-K4
Product Type Tetramer
Size 25 µg
Application FCM
Price
$1,500.00
Qty: