QuickSwitch™ Quant H-2 Kb Tetramer Kit-PE
Specifications:
Background
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Description
QuickSwitch™ Tetramer Kit utilizes a patented technique for exchanging up to ten peptides on an MHC class I tetramer. Reduce time and cost with this kit to create your own custom tetramers or to test which peptide sequence has the best peptide-MHC binding affinity. This kit can be used for screening multiple peptides or to create a small amount of your own custom Tetramer in house. The resulting custom tetramers can be used for multiple applications including screening immunogenic peptides, neoantigen discovery, and many other assays downstream from in-silico selection.
FAQs for Peptide Exchange using QuickSwitch™ Kit
| Target: | Custom |
|---|---|
| Product Type: | Tetramer |
| Size: | 1 kit |
| Application: | FCM |
| Research Area / Disease: | Immune Monitoring, Immunology |
| Class: | Class I |
| Allele: | H-2Kb |
| Peptide Sequence: | Custom |
| Conjugate: | PE |
| Species Reactivity: | Mouse |
| Specificity: | Custom |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
Citations
Marc C Delcommenne, Olga Hrytsenko, Cynthia Tram, Genevieve Weir and Marianne M. Stanford. (2017) The QuickSwitch Quant HLA-A*02:01 Tetramer Kit can be used for determining the biological activity of a cancer vaccine. J Immunol, 198 (1 Supplement) 79.27; https://www.jimmunol.org/content/198/1_Supplement/79.27
Song, X., Lu, Z., & Xu, J. (2020). Targeting cluster of differentiation 47 improves the efficacy of anti‑cytotoxic T‑lymphocyte associated protein 4 treatment via antigen presentation enhancement in pancreatic ductal adenocarcinoma. Experimental and Therapeutic Medicine. doi:10.3892/etm.2020.9054
Najafabadi, A. H., Zhang, J., Aikins, M. E., Abadi, Z. I., Liao, F., Qin, Y., Moon, J. J. (2020). Cancer Immunotherapy via Targeting Cancer Stem Cells Using Vaccine Nanodiscs. Nano Letters, 20(10), 7783-7792. doi:10.1021/acs.nanolett.0c03414
Son, E. T., Faridi, P., Paul-Heng, M., Leong, M., English, K., Ramarathinam, S. H., . . . Sharland, A. F. (2020). The self-peptide repertoire plays a critical role in transplant tolerance induction. Biorxiv. doi:10.1101/2020.11.09.359968
Sydney X. Lu, Emma de Neef, James D. Thomas, Luis Diaz, Jr., Omar Abdel-Wahab, Robert K. Bradley. (2021) Pharmacologic modulation of RNA splicing enhances anti-tumor immunity. Cell, 12066. doi: /10.1016/j.cell.2021.05.038
Son, Eric T., et al. “Screening self-peptides for recognition by mouse alloreactive CD8+ T cells using direct ex vivo multimer staining.” STAR protocols 4.1 (2023): 101943. doi.org/10.1016/j.xpro.2022.101943
References
Kula, T., Dezfulian, M. H., Wang, C. I., Abdelfattah, N. S., Hartman, Z. C., Wucherpfennig, K. W., … Elledge, S. J. (2019). T-Scan: A Genome-wide Method for the Systematic Discovery of T Cell Epitopes. Cell, 178(4). doi: 10.1016/j.cell.2019.07.009
Christof C. Smith, Shengjie Chai, Amber R. Washington, Samuel J. Lee, Elisa Landoni, Kevin Field, Jason Garness, Lisa M. Bixby, Sara R. Selitsky, Joel S. Parker, Barbara Savoldo, Jonathan S. Serody, and Benjamin G. Vincent (2019) Machine-Learning Prediction of Tumor Antigen Immunogenicity in the Selection of Therapeutic Epitopes doi: 10.1158/2326-6066.CIR-19-0155
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