Products MHC Tetramers & Monomers QuickSwitch™ Custom Tetramer Kits QuickSwitch™ Quant H-2 Kb Tetramer Kit-PE

QuickSwitch™ Quant H-2 Kb Tetramer Kit-PE

Specifications:

Background

Description

QuickSwitch™ Tetramer Kit utilizes a patented technique for exchanging up to ten peptides on an MHC class I tetramer. Reduce time and cost with this kit to create your own custom tetramers or to test which peptide sequence has the best peptide-MHC binding affinity. This kit can be used for screening multiple peptides or to create a small amount of your own custom Tetramer in house. The resulting custom tetramers can be used for multiple applications including screening immunogenic peptides, neoantigen discovery, and many other assays downstream from in-silico selection.

FAQs for Peptide Exchange using QuickSwitch™ Kit

Target:	Custom
Product Type:	Tetramer
Size:	1 kit
Application:	FCM
Research Area / Disease:	Immune Monitoring, Immunology
Class:	Class I
Allele:	H-2Kb
Peptide Sequence:	Custom
Conjugate:	PE
Species Reactivity:	Mouse
Specificity:	Custom

Citations

Wang, Wenfeng, et al. “Lymphatic endothelial transcription factor Tbx1 promotes an immunosuppressive microenvironment to facilitate post-myocardial infarction repair.” Immunity (2023).

Son, Eric T., et al. “Screening self-peptides for recognition by mouse alloreactive CD8+ T cells using direct ex vivo multimer staining.” STAR protocols 4.1 (2023): 101943. doi.org/10.1016/j.xpro.2022.101943

Hocheol Shin, Yujin Kim, and Sangyong Jon ACS Nano 2023 17 (11), 10337-10352 DOI: 10.1021/acsnano.3c00764ao,

Sydney X. Lu, Emma de Neef, James D. Thomas, Luis Diaz, Jr., Omar Abdel-Wahab, Robert K. Bradley. (2021) Pharmacologic modulation of RNA splicing enhances anti-tumor immunity. Cell, 12066. doi: /10.1016/j.cell.2021.05.038

Song, X., Lu, Z., & Xu, J. (2020). Targeting cluster of differentiation 47 improves the efficacy of anti‑cytotoxic T‑lymphocyte associated protein 4 treatment via antigen presentation enhancement in pancreatic ductal adenocarcinoma. Experimental and Therapeutic Medicine. doi:10.3892/etm.2020.9054

Najafabadi, A. H., Zhang, J., Aikins, M. E., Abadi, Z. I., Liao, F., Qin, Y., Moon, J. J. (2020). Cancer Immunotherapy via Targeting Cancer Stem Cells Using Vaccine Nanodiscs. Nano Letters, 20(10), 7783-7792. doi:10.1021/acs.nanolett.0c03414

Son, E. T., Faridi, P., Paul-Heng, M., Leong, M., English, K., Ramarathinam, S. H., . . . Sharland, A. F. (2020). The self-peptide repertoire plays a critical role in transplant tolerance induction. Biorxiv. doi:10.1101/2020.11.09.359968

Marc C Delcommenne, Olga Hrytsenko, Cynthia Tram, Genevieve Weir and Marianne M. Stanford. (2017) The QuickSwitch Quant HLA-A*02:01 Tetramer Kit can be used for determining the biological activity of a cancer vaccine. J Immunol, 198 (1 Supplement) 79.27; https://www.jimmunol.org/content/198/1_Supplement/79.27

References

Kula, T., Dezfulian, M. H., Wang, C. I., Abdelfattah, N. S., Hartman, Z. C., Wucherpfennig, K. W., … Elledge, S. J. (2019). T-Scan: A Genome-wide Method for the Systematic Discovery of T Cell Epitopes. Cell, 178(4). doi: 10.1016/j.cell.2019.07.009

Christof C. Smith, Shengjie Chai, Amber R. Washington, Samuel J. Lee, Elisa Landoni, Kevin Field, Jason Garness, Lisa M. Bixby, Sara R. Selitsky, Joel S. Parker, Barbara Savoldo, Jonathan S. Serody, and Benjamin G. Vincent (2019) Machine-Learning Prediction of Tumor Antigen Immunogenicity in the Selection of Therapeutic Epitopes doi: 10.1158/2326-6066.CIR-19-0155