Products MHC Tetramers & Monomers QuickSwitch™ Custom Tetramer Kits QuickSwitch™ Quant HLA-A*01:01 Tetramer Kit-BV421

QuickSwitch™ Quant HLA-A*01:01 Tetramer Kit-BV421

Specifications:

Background

Description

QuickSwitch™ Tetramer Kit utilizes a patented technique for exchanging up to ten peptides on an MHC class I tetramer. Reduce time and cost with this kit to create your own custom tetramers or to test which peptide sequence has the best peptide-MHC binding affinity. This kit can be used for screening multiple peptides or to create a small amount of your own custom Tetramer in house. The resulting custom tetramers can be used for multiple applications including screening immunogenic peptides, neoantigen discovery, and many other assays downstream from in-silico selection.

FAQs for Peptide Exchange using QuickSwitch™ Kit

Target:	Custom
Product Type:	Tetramer
Size:	1 kit
Application:	FCM
Research Area / Disease:	Immune Monitoring, Immunology
Class:	Class I
Allele:	HLA-A*01:01
Peptide Sequence:	Custom
Conjugate:	BV421
Species Reactivity:	Human
Specificity:	Custom
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

Citations

Reaper DR, Cresswell P. 2008. Regulation of MHC class I assembly and peptide binding. Annu Rev Cell Dev Biol. 24:343-368.
Mayerhofer PU, Tampé R. 2015. Antigen translocation machineries in adaptive immunity and viral immune evasion. J Mol Biol. 2015 427(5):1102-1118.
Wooldridge L, van den Berg HA, Glick M, Gostick E, Laugel B, Hutchinson SL, Milicic A, Brenchley JM, Douek DC, Price DA, Sewell AK. 2005. Interaction between the CD8 coreceptor and major histocompatibility complex class I stabilizes T cell receptor–antigen complexes at the cell surface. J Biol Chem 280:27491–501.
Wooldridge L, Lissina A, Cole DK, van den Berg HA, Price DA, Sewell AK. 2009. Tricks with tetramers: how to get the most from multimeric peptide-MHC. Immunology. Feb;126(2):147-64.
Price DA , Brenchley JM, Ruff LE, Betts MR, Hill BJ, Roederer M, Koup RA, Migueles SA, Gostick E, Wooldridge L, Sewell AK, Connors M, Douek DC . 2005. Avidity for antigen shapes clonal dominance in CD8 T cell populations specific for persistent DNA viruses. J. Exp. Med. 202:1349-1361.
Mielke S, Nunes R, Rezvani K, et al. 2008. A clinical-scale selective allodepletion approach for the treatment of HLA-mismatched and
matched donor-recipient pairs using expanded T lymphocytes as antigen-presenting cells and a TH9402-based photodepletion technique. Blood. 111:4392-4402