QuickSwitch™ HLA-A*02:01 Quant Monomer Kit
Specifications:
Background
The QuickSwitch™ Quant Monomer Kit utilizes a patented technique for exchanging up to ten peptides on an MHC Class I Monomer. After performing your peptide exchange, you can tetramerize the monomers and add your own conjugate. Create your own custom Class I MHC Tetramers in your own lab.
Description
The QuickSwitch™ Quant Monomer Kit utilizes a patented technique for exchanging up to ten peptides on an MHC Class I Monomer. Components for quantifying the extent of peptide exchange by flow cytometry are included in this kit.
| Product Type: | Monomer |
|---|---|
| Size: | 1 kit |
| Application: | FCM |
| Research Area / Disease: | Immune Monitoring, Immunology |
| Class: | Class I |
| Allele: | HLA-A*02:01 |
| Peptide Sequence: | Custom |
| Species Reactivity: | Human |
| Specificity: | Custom |
Components
- QuickSwitch™ HLA-A*02:01 Monomer
- Peptide Exchange Factor # 1
- Magnetic Capture Beads
- Exiting Peptide Antibody-FITC (1x)
- Reference Peptide 1 mM
- Assay Buffer (10x)
References
1. Reaper DR, Cresswell P. 2008. Regulation of MHC class I assembly and peptide binding. Annu Rev Cell Dev Biol. 24:343-368.
2. Mayerhofer PU, Tampé R. 2015. Antigen translocation machineries in adaptive immunity and viral immune evasion. J Mol Biol. 2015 427(5):1102-1118.
3. Altman J. D., Davis M. M. MHC-peptide tetramers to visualize antigen-specific T cells. Curr. Protoc. Immunol. 115, 17 13 11-17 13 44 (2016).
Related Content:
Videos
Video: MBL's MHC Tetramers
