Products Research Area/Disease Epigenetics RIP-Assay Kit　for microRNA

RIP-Assay Kit　for microRNA

RIP-Assay Kit.

Specifications:

Description

RIP-Assay Kit for microRNA is optimized to immunochemically isolate endogenous miRNAs, mRNAs and RBPs within mRNP complexes. The kit is designed to isolate cellular miRNAs that being incorporated into the RISC and/or to isolate unique group of miRNAs that bind to specific group of mRNAs encoding functionally related proteins.

Target:	RNP Complex
Product Type:	Kit
Size:	10 Assays
Research Area / Disease:	Epigenetics
Storage Temperature:	4°C
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

Components

mi-Lysis Buffer
mi-Wash Buffer
Normal Rabbit IgG
High-Salt Solution
mi-Solution I
mi-Solution II
mi-Solution III
mi-Solution IV
Gel Extraction Buffer
3M NaOAc
miSPIKETM

Citations

Comincini S et al. microRNA-17 regulates the expression of ATG7 and modulates the autophagy process, improving the sensitivity to temozolomide and low-dose ionizing radiation treatments in human glioblastoma cells. Cancer Biol Ther. 14, 574-86 (2013),
Degrauwe N et al. The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Cell Rep. 15, 1634-47 (2016),
Fu A et al. Targetome profiling and functional genetics implicate miR-618 in lymphomagenesis. Epigenetics 9, 730-7 (2014),
He T et al. MicroRNA-542-3p inhibits tumour angiogenesis by targeting angiopoietin-2. J Pathol. 232, 499-508 (2014),
Hoffman AE et al. Targetome profiling, pathway analysis and genetic association study implicate miR-202 in lymphomagenesis. Cancer Epidemiol Biomarkers Prev. 22, 327-36 (2013),
Madhyastha R et al. NFkappaB activation is essential for miR-21 induction by TGFβ1 in high glucose conditions. Biochem Biophys Res commun. 451, 615-21 (2014)
,
Marchesi N et al. Autophagy is modulated in human neuroblastoma cells through direct exposition to low frequency electromagnetic fields. J Cell Physiol. 229, 1776-86 (2014),
Uchino K et al. Therapeutic Effects of MicroRNA-582-5p and -3p on the Inhibition of Bladder Cancer Progression. Mol Ther. 21, 610-9 (2013)
The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Derauwe N et al. Cell Rep. (2016)
Bach DH et al. BMP4 Upregulation Is Associated with Acquired Drug Resistance and Fatty Acid Metabolism in EGFR-Mutant Non-Small-Cell Lung Cancer Cells. Mol Ther Nucleic Acids. 12, 817-828 (2018)
Wen J et al. miR-424 coordinates multilayered regulation of cell cycle progression to promote esophageal squamous cell carcinoma cell proliferation. EBioMedicine. 37, 110-124 (2018)rn