MESACUP BP230 TEST

  • Applications
    • ELISA
  • Code # RG-M7613-D
  • Size 48 wells
  • Price Call for Price
Specifications

Background

Bullous pemphigoid (BP) is chronic itchy blistering disorder found mainly in aged persons, characterized by frequent occurring of tense blister and erythema. IgG anti-basement membrane zone (BMZ) antibodies are found in the serum of patients, and linear IgG or C3 sediment is found on the basement membrane zone of the lesion. Target antigens of the autoantibodies in BP patient serum are BP180 and BP230, also called BPAG1 and BPAG2. Molecular weight of these antigens is 230 kD and 180 kD respectively. Anti-BP180 is thought to be the pathogenic autoantibody, however, not all BP patients have anti-BP180 antibody in their serum. Anti-BP230 antibody is considered to be a useful serologic marker of the disease.

The MESACUP BP230 TEST has recombinant protein of both N-terminus and C-terminus of BP230 as solid phase and measures anti-BP230 autoantibodies in patient serum.

  • Application:
    ELISA
  • Components:
    1. BP180 Microwell Strips 48 wells Microwell strips (6 x 8 wells) coated with recombinant purified BP180NC16a antigen. The breakaway strips are packed in a strip holder and sealed in a foil envelope with desiccant.Stable at 2-8ºC until labeled expiration date.
    2. Calibrator 1 (0 U/mL) One vial containing 1.5 mL of assay diluent including 0.09% sodium azide. Stable at 2-8ºC until labeled expiration date.
    3. Calibrator 2 (100 U/mL) One vial containing 1.5 mL of anti-BP180 positive antibody with assay diluent including 0.09% sodium azide. Stable at 2-8ºC until labeled expiration date.
    4. Conjugate Reagent One vial containing 8 mL of horseradish peroxidase conjugated goat anti-human IgG antibody. Stable at 2-8ºC until labeled expiration date.
    5. Assay Diluent One vial containing 50 mL of PBS, Tween 20 and 0.09% sodium azide. Stable at 2-8ºC until labeled expiration date.
    6. Wash Concentrate (10X) One vial containing 100 mL of PBS and Tween 20 as a 10X concentrate. Stable at 2-8ºC until labeled expiration date.
    7. Substrate One vial containing 20 mL of 3,3',5,5'-tetramethylbenzidine dihydrochloride/hydrogen peroxide (TMB/H2O2).Stable at 2-8ºC until labeled expiration date.
    8. Stop Solution One vial containing 20 mL of 1.0N sulfuric acid. Stable at 2-8ºC until labeled expiration date.
  • Description:

    INTENDED USE

    The MESACUP BP230 TEST is a semi-quantitative Enzyme-Linked Immunosorbent Assay (ELISA) for the detection of anti BP230 antibodies in human serum. The MESACUP BP230 TEST is intended for in vitro diagnostic use as an aid in the diagnosis of bullous pemphigoid in conjunction with other laboratory and clinical findings. Patients with Bullous Pemphigoid are known to have either BP180 or BP230 or both types of antibodies in serum. It is recommended that each patient be tested for BP180 and BP230 antibodies.

  • Product Type:
    ELISA Kit
  • Research Area:
    Autoimmune
  • Short Description:

    INTENDED USE

    The MESACUP BP230 TEST is a semi-quantitative enzyme-linked immunosorbent assay (ELISA) for the detection of anti BP180 antibodies in human serum. The MESACUP BP230 TEST is intended for in vitro diagnostic use as an aid in the diagnosis of bullous pemphigoid in conjunction with other laboratory and clinical findings. Patients with Bullous Pemphigoid are known to have either BP180 or BP230 or both types of antibodies in serum. It is recommended that each patient be tested for BP180 and BP230 antibodies.

  • Size:
    48 wells
References
  1. Stanley JR, Hawley-Nelson P, Yuspa SH, Shevach EM, Katz SI: Characterization of bullous pemphigoid antigen: A unique basement membrane protein of stratified aquamous epithelia. Cell 24: 897-903, 1981.
  2. Thoma-Uszynski S, Uter W, Schwietzke S, Hofmann SC, Hunziker T, et. Al.: BP230- and BP180-specific auto-antibodies in bullous pemphigoid. J Invest Dermatol. 122:1413-22, 2004.
  3. Hamada T, Nagata Y, Tomita M, Salmhofer W, Hashimoto T: Bullous pemphigoid sera react specifically with various domains of BP230, most frequently with C-terminal domain, by immunoblot analysis using bacterial recombinant proteins covering the entire molecule. Exp Dermatol 10: 256-263, 2001.
  4. Yoshida M, Hamada T, Amagai M, et al.: Enzyme-linked immunosorbent assay using bacterial recombinant proteins of human BP230 as a diagnostic tool for bullous pemphigoid. J Dermatol Sci. 41:21-30, 2006.