MESACUP BP180 TEST

  • Applications
    • ELISA
  • Code # RG-M7612-D
  • Size 48 wells
  • Price Call for Price
Specifications

Background

Bullous pemphigoid (BP) is a chronic itchy blistering skin disorder found mainly in the elderly population and is characterized by frequent occurring of tense blisters and erythema. IgG antibasement membrane zone (BMZ) antibodies are found in the serum of patients, and linear IgG or C3 sediment is found on the basement membrane zone of the lesion. Target antigens of the autoantibodies in BP patient serum are BP230 and BP180, also called BPAG1 and BPAG2. Molecular weight of these antigens is 230 kD and 180 kD respectively.

BP180 is thought to be the direct target of the autoantibody because of its location, and the autoantibodies against BP230 are thought to be secondarily produced.The antibodies against BP180 are thought to be pathogenic, because the rabbit antibody against mouse in the NC16a region of BP180 forms blisters similar to BP when injected to neonatal mice. The main epitope of BP180 is located in the region close to cell membrane called NC16a and most patient serum reacts with the recombinant NC16a protein. The MESACUP BP180 TEST is for measuring anti BP180 autoantibodies in patient serum.

  • Application:
    ELISA
  • Components:
    1. BP180 Microwell Strips 48 wells Microwell strips (6 x 8 wells) coated with recombinant purified BP180NC16a antigen. The breakaway strips are packed in a strip holder and sealed in a foil envelope with desiccant. Stable at 2-8ºC until labeled expiration date.
    2. Calibrator 1 (0 U/mL) One vial containing 1.5 mL of assay diluent including 0.09% sodium azide. Stable at 2-8ºC until labeled expiration date.
    3. Calibrator 2 (100 U/mL) One vial containing 1.5 mL of anti-BP180 positive antibody with assay diluent including 0.09% sodium azide. Stable at 2-8ºC until labeled expiration date.
    4. Conjugate Reagent One vial containing 8 mL of horseradish peroxidase conjugated goat anti-human IgG antibody. Stable at 2-8ºC until labeled expiration date.
    5. Assay Diluent One vial containing 50 mL of PBS, Tween 20 and 0.09% sodium azide. Stable at 2-8ºC until labeled expiration date.
    6. Wash Concentrate (10X) One vial containing 100 mL of PBS and Tween 20 as a 10X concentrate. Stable at 2-8ºC until labeled expiration date.
    7. Substrate One vial containing 20 mL of 3,3',5,5'-tetramethylbenzidine dihydrochloride/hydrogen peroxide (TMB/H2O2).Stable at 2-8ºC until labeled expiration date.
    8. Stop Solution One vial containing 20 mL of 1.0N sulfuric acid. Stable at 2-8ºC until labeled expiration date.
  • Description:

    INTENDED USE

    The MESACUP BP180 TEST is a semi-quantitative Enzyme-Linked Immunosorbent Assay (ELISA) for the detection of anti BP180 antibodies in human serum. The MESACUP BP180 TEST is intended for in vitro diagnostic use as an aid in the diagnosis of bullous pemphigoid in conjunction with other laboratory and clinical findings. Patients with Bullous Pemphigoid are known to have either BP180 or BP230 or both types of antibodies in serum. It is recommended that each patient be tested for BP180 and BP230 antibodies.

     

  • Product Type:
    ELISA Kit
  • Research Area:
    Autoimmune
  • Short Description:

    INTENDED USE

    The MESACUP BP180 TEST is a semi-quantitative enzyme-linked immunosorbent assay (ELISA) for the detection of anti BP180 antibodies in human serum. The MESACUP BP180 TEST is intended for in vitro diagnostic use as an aid in the diagnosis of bullous pemphigoid in conjunction with other laboratory and clinical findings. Patients with Bullous Pemphigoid are known to have either BP180 or BP230 or both types of antibodies in serum. It is recommended that each patient be tested for BP180 and BP230 antibodies.

  • Size:
    48 wells
References
  1. Stanley JR, Hawley-Nelson P, Yuspa SH, Shevach EM, Katz SI : Characterization of bullous pemphigoid antigen: A unique basement membrane protein of stratified aquamous epithelia.Cell 24 : 897-903, 1981.
  2. Liu Z, et al.: A passive transfer model of the organ-specific autoimmune disease, bullous pemphigoid, using antibodies generated against the hemidesmosomal antigen, BP180. J Clin Invest 92:2480-2488, 1993.
  3. Matsumura K, et al.: The majority of bullous pemphigoid and herpes gestationis serum samples;react withNC16a domain of the 180kD bullous pemphigoid antigen. Arch Dermatol Res 288:507-509, 1996.
  4. Yoshida M, Hamada T, Amagai M, et al.: Enzyme-linked immunosorbent assay using bacterial recombinant proteins of human BP230 as a diagnostic tool for bullous pemphigoid. J Dermatol Sci. 41:21-30, 2006.