The MBL Bion DSG 1 & DSG 3 ELISA TEST SYSTEM is a semi-quantitative Enzyme-Linked Immunosorbent Assay for the detection of autoantibodies to desmoglein 1 (DSG 1) and desmoglein 3 (DSG 3) in human serum. The MBL Bion DSG 1 & DSG 3 ELISA TEST SYSTEM is intended for in vitro diagnostic use as an aid in the diagnosis of certain pemphigus diseases.
Pemphigus includes a group of often fatal, autoimmune, blistering diseases characterized by intraepithelial lesions. Pemphigus vulgaris and its variants may present with oral or other mucosal lesions alone or with mucosal plus skin lesions. Pemphigus foliaceus and its variants present with skin lesions alone. Indirect immunofluorescent (IIF) studies reveal that both forms of pemphigus are caused by autoantibodies to cell surface antigens of stratified epithelia of mucous membranes and skin. These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1 (DSG 1) in pemphigus foliaceus and desmoglein 3 (DSG 3) in pemphigus vulgaris. Pemphigus vulgaris patients with both mucosal and skin lesions have antibodies to both DSG 3 and DSG 1.
The diagnosis of pemphigus depends on biopsy and serum studies that characterize lesions and detect the autoantibodies that cause them. Serum studies afford highly sensitive diagnostic aids. Originally they were performed by indirect immunofluorescence (IIF) using monkey esophagus and other tissues sections. The identification of the reactive antigens as DSG 1 and DSG 3 has made it possible to develop highly specific and sensitive ELISA methods.
The MBL Bion DSG 1 & DSG 3 ELISA TEST SYSTEM is a semi-quantitative enzyme-linked immunosorbent assay for the detection of autoantibodies to desmoglein 1 (DSG 1) and desmoglein 3 (DSG 3) in human serum. The MBL Bion DSG 1 & DSG 3 ELISA TEST SYSTEM is intended for in vitro diagnostic use as an aid in the diagnosis of certain pemphigus diseases.