DSG 1 & 3 ELISA Test System

  • Applications
    • ELISA
  • Code # RG-M7593-D
  • Size 96 tests
  • Price Call for Price
Specifications

Background

Pemphigus includes a group of often fatal, autoimmune, blistering diseases characterized by intraepithelial lesions. Pemphigus vulgaris and its variants may present with oral or other mucosal lesions alone or with mucosal plus skin lesions. Pemphigus foliaceus and its variants present with skin lesions alone. Indirect immunofluorescent (IIF) studies reveal that both forms of pemphigus are caused by autoantibodies to cell surface antigens of stratified epithelia of mucous membranes and skin. These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1 (DSG 1) in pemphigus foliaceus and desmoglein 3 (DSG 3) in pemphigus vulgaris. Pemphigus vulgaris patients with both mucosal and skin lesions have antibodies to both DSG 3 and DSG 1. 

The diagnosis of pemphigus depends on biopsy and serum studies that characterize lesions and detect the autoantibodies that cause them. Serum studies afford highly sensitive diagnostic aids. Originally they were performed by indirect immunofluorescence (IIF) using monkey esophagus and other tissues sections. The identification of the reactive antigens as DSG 1 and DSG 3 has made it possible to develop highly specific and sensitive ELISA methods.

 

 

  • Application:
    ELISA
  • Components:
    • DSG 1 MICROWELL STRIPS 48 wells coated with antigen produced from recombinant purified proteins of Desmoglein 1 (DSG 1) antigens (6 x 8 well strips). The breakaway strips packaged in a strip holder and sealed in a foil envelope with desiccant, are stable at 2-8°C Unused strips are stable for 60 days stored at the properly resealed envelope.
    • DSG 3 MICROWELL STRIPS 48 wells coated with antigen produced from recombinant purified proteins of Desmoglein 3 (D SG 3) antigen (6 x 8 well strips). The breakaway strips packaged in a strip holder and sealed in a foil envelope with desiccant, are stable at 2-8°C. Unused strips are stable for 60 days stored at 2-8 C in the properly resealed envelope. Color-coded with a red stripe for identification and differentiation from DSG-1 microwells.
    • DSG CONJUGATE CONCENTRATE (101X) One vial containing 0.3 mL of horseradish peroxidase conjugated mouse monoclonal anti-human IgG (Fab) at a 101x concentration. Stable at 2-8 C until labeled expiration date.
    • CONJUGATE DILUENT One bottle containing 24 mL of HEPES, saline, Bromphenol Blue, p-Hydroxyphenylacetic acid, Proclin 150, and BSA at a pH of 7.4 +0.2. Ready to use. Stable at 2-8 C until labeled expiration date.
    • NEGATIVE CALIBRATOR One vial containing 1.5 mL of normal human serum with Tris-HCl, NaCl, CaCl2, bovine serum and sodium azide of Sample Diluent. Stable at 2-C until labeled expiration date.
    • DSG 1 CALIBRATOR One vial containing 1.5 mL of anti-DSG 1 antibody positive human serum with Tris- HCl, NaCl, CaCl2, bovine serum and sodium azide. Stable at 2-8 C until labeled expiration date.
    • DSG 3 CALIBRATOR One vial containing 1.5 mL of anti-DSG 3 antibody positive human serum with Tris- HCl, NaCl, CaCl2, bovine serum and sodium azide. Stable at 2-8 C until labeled expiration date.
    • SAMPLE DILUENT Two 50 mL bottles containing Tris- HCl, NaCl, CaCl2, bovine serum, high five cell extract and sodium azide at a pH of of 7.4 + 0.2. Ready to use. Stable at 2-8 C until labeled expiration date.
    • WASH BUFFER CONCENTRATE (10X) One 100 mL bottle containing sodium dihydrogen phosphate, sodium chloride and Tween-20 as a 10X concentrate. Stable at 2-8 C until labeled expiration date. The 1X solution is at a pH of 7.4 + 0.2.SUBSTRATE SOLUTION One 20 mL bottle containing 3,3',5,5'-tetramethylbenzidine dihydrochloride/hydrogen peroxide (TMB/H2O2).
    • Ready to use. Stable at 2-8C until labeled expiration date.
    • STOP SOLUTION One 20 mL bottle containing 1.0N sulfuric acid solution, ready to use. Stable at 2-8 C until labeled expiration date. 
  • Description:

    INTENDED USE

    The MBL Bion DSG 1 & DSG 3 ELISA TEST SYSTEM is a semi-quantitative Enzyme-Linked Immunosorbent Assay for the detection of autoantibodies to desmoglein 1 (DSG 1) and desmoglein 3 (DSG 3) in human serum. The MBL Bion DSG 1 & DSG 3 ELISA TEST SYSTEM is intended for in vitro diagnostic use as an aid in the diagnosis of certain pemphigus diseases.

     

  • Product Type:
    ELISA Kit
  • Research Area:
    Autoimmune
  • Short Description:

    INTENDED USE

    The MBL Bion DSG 1 & DSG 3 ELISA TEST SYSTEM is a semi-quantitative enzyme-linked immunosorbent assay for the detection of autoantibodies to desmoglein 1 (DSG 1) and desmoglein 3 (DSG 3) in human serum. The MBL Bion DSG 1 & DSG 3 ELISA TEST SYSTEM is intended for in vitro diagnostic use as an aid in the diagnosis of certain pemphigus diseases.

  • Size:
    96 tests
References
  1. Lever WF. Pemphigus. Medicine 1953; 32:1-123.
  2. Lever WF. Pemphigus and Pemphigoid. Charles Thomas. Springfield, IL 1965.
  3. Beutner EH, Jordon RE, Chorzelski TP. The immunopathology of pemphigus and bullous pemphigoid. J Invest Dermatol 1968; 51:63-80.
  4. Beutner EH, Chorzelski TP, Jablonska S. Clinical significance of immunofluorescent tests of sera and skin in bullous diseases: A cooperative study. In “Immunopathology of the skin” (EH Beutner, TP Chorzelski and V Kumar ed.) 3rd Ed. 1987, 177-205.
  5. Stanley JR, Koulu L, Thivolet C. Distinction between epidermal antigens binding pemphigus vulgaris autoantibodies and pemphigus foliaceus autoantibodies. J Clin Invest 1984, 74:313-320.
  6. Stanley JR. Cell adhesion molecules as targets of autoantibodies in pemphigus and pemphigoid, bullous disease due to defective epidermal adhesion. Advances in Immunol 1992, 53:291-325.
  7. Amagai M, Tsunoda K, Zillikens D, Nagai T, Nishikawa T. The clinical phenotype of pemphigus is defined by the anti-desmoglein autoantibody profile. J Am Acad Dermatol 1999, 40:167-70.
  8. Sabolinski ML, Beutner EH, Krasny S, Kumar V, Huang J, Chorzelski TP, et al. Substrate specificity of antiepithelial antibodies of pemphigus vulgaris and pemphigus foliaceus sera in immunofluorescence tests on monkey and guinea pig esophagus sections. J Invest Dermatol 1987, 88:545-49.
  9. Amagai M, Komai A, Hashimoto T, Shirakata Y, Hashimoto K, Yamada T, et al. Usefulness of enzyme linked immunosorbent assay using recombinant desmogleins 1 and 3 for sero-diagnosis of pemphigus. Brit J Dermatol 1999, 140: 351-357.
  10. Ishii K, Amagai M, Hall RP, Hashimoto T, Takayanagi A, et al. Characterization of autoantibodies in pemphigus using antigen specific Elisa’s with baculovirus expressed recombinant desmogleins. J Immunol 1997, 159: 2010-2017.
  11. Harman KE, Gratin MJ, Bhogal SJ, Challecomebe SJ, Black M. The clinical significance of autoantibodies to desmoglein 1 in 78 cases of pemphigus vulgaris. J Invest Derm  1999, 112:568 (Abstr).
  12. Data on file at MBL Bion, Inc.