Anti-MDA5 (RUO)

  • Applications
    • ELISA
  • Code # RG-7873R
  • Size 48 wells
  • Price Call for Price
Specifications

Background

MDA5, also known as CADM-140 is one of the retinoic acid inducible gene I(RIG-I)family proteins and involved in innate immune defense against viruses, cellular growth suppression, and apoptosis. And these responses also could trigger the autoimmune response to MDA5.
The Anti-MDA5 ELISA Kit is for measuring anti-MDA5 antibody specifically with high sensitivity in ELISA.

  • Application:
    ELISA
  • Components:


    1) MDA5 Microwell strips
    48 wells Microwell strips (8 x 6 wells) coated with recombinant MDA5 antigen. The breakaway strips are packed in a strip holder and sealed in a ziplock pouch with a desiccant.
    2) Calibrator 1 (0 U/mL)
    One vial containing 1.5 mL of assay diluent containing 0.09% sodium azide. Ready to use, make no further dilution.
    3) Calibrator 2 (100 U/mL)
    One vial containing 1.5 mL of human serum in assay diluent containing 0.09% sodium azide. Ready to use, make no further dilution.
    4) Conjugate
    One vial containing 8 mL of horseradish peroxidase conjugated goat anti-human IgG antibody and ProClin 150. Ready to use, make no further dilution.
    5) Assay diluent
    One vial containing 50 mL of PBS, containing Tween 20 and 0.09% sodium azide. Ready to use, make no further dilution.
    6) Wash buffer (20x)
    One vial containing 50 mL of PBS with Tween 20 concentrate (20x).
    7) Substrate
    One vial containing 20 mL of 3,3',5,5'-tetramethylbenzidine dihydrochloride/hydrogen peroxide (TMB/ H2O2). Ready to use, make no further dilution.
    8) Stop solution
    One vial containing 20 mL of 0.25 mol/L sulfuric acid. Ready to use, make no further dilution.
    9) High control
    One vial containing 1.5 mL of human serum in assay diluent containing 0.09% sodium azide. Ready to use, make no further dilution.
    10) Low control
    One vial containing 1.5 mL of human serum in assay diluent containing 0.09% sodium azide. Ready to use, make no further dilution.
    Unopened kit components are stable until the labeled expiration date at 2-8°C.

  • Description:

    Anti-MDA5 ELISA Kit is a semi-quantitative enzyme-linked immunosorbent assay (ELISA) for the detection of anti-melanoma differentiation associated gene 5 protein (MDA5) antibodies in human serum. Anti-MDA5 ELISA Kit is intended for research use only. Not for use in diagnostic procedures.

  • Product Type:
    ELISA Kit
  • Research Area:
    Autoimmune
  • Short Description:

    Anti-Synthetase ELISA kit is an Enzyme Linked Immuno-Sorbent assay (ELISA) detection kit for anti-aminoacyl-tRNA synthetase antibodies in serum.

    For Research Use Only. Not for use in diagnostic procedures.

  • Size:
    48 wells
References
  1. Nishikai M, Reichlin M: Heterogeneity of precipitating antibodies in polymyositis and dermatomyositis: characterization of Jo-1 antibody system. Arthritis Rheum 23: 881-888, 1980
  2. Targoff IN, Arnett FC, Reichlin M: Antibody to threonyl-transfer RNA synthetase in mypsitis sera. Arthritis Rheum 31: 515-524, 1988
  3. Hirakata M, Suwa A, Nagai S, Korn MA, Trieu EP, Mimori T, Aizuki M, Targoff IN: Anti-KS: Identification of autoantibodies to asparaginyl-transfer RNA synthetase associated with interstitial lung disease. J. Immunol. 162: 2315-2320, 1999
  4. Targoff IN, Trieu EP, Plotz PH, Miller FW: Antibodies to glycyl-transfer RNA synthetase in patients with myositis and interstitial lung disease. Arthritis Rheum 35: 821-820, 1992
  5. Targoff IN: Autoantibodies to aminoacyl-transfer RNA synthetases for isoleucine and glycine. J. Immunol. 144: 1737-1743, 1990.