Anti-Type VII collagen ELISA Kit (RUO)

  • Applications
    • ELISA
  • Code # RG-7845R2
  • Size 48 wells
  • Price Call for Price
Specifications

Background

Epidermolysis bullosa aquisita (EBA) is an autoimmune blistering skin disease characterized by the presence of Type VII Collagen IgG autoantibodies. Type VII collagen is a major component of anchoring fibrils, attachment structures within the basement membrane, between the epidermis and dermis of human skin. Type VII collagen is composed of three identical α-chains. Each α-chain consists of a central collagenus (COL) domain flanked by a 145 kDa non-collagenous amino-terminal (NC1) domain, and a smaller 34 kDa carboxyl-terminal non-collagen (NC2) domain.

The major antigenic epitopes for anti-Type VII collagen autoantibodies in patients with EBA reside within the NC1 domain. However, the recent research reports that NC2 domain is a minor antigenic epitopes. To develop an ELISA assay with the highest sensitivity to identify Type VII collagen antibodies, two purified recombinant antigens (NC1 and NC2) were combined and coated in the same well of ELISA microplate. This ELISA system is able to detect anti-type VII collagen autoantibodies containing not only anti-NC1antibody but also anti-NC2 antibody.

  • Application:
    ELISA
  • Components:
    • Type VII collagen Microwell Strips: 8-wells x 6 strips
    • Calibrator 1 (0 U/mL): 1.5 mL x 1 vial
    • Calibrator 2 (100 U/mL): 1.5 mL x 1 vial
    • Conjugate Reagent (Peroxidase-conjugated anti-human IgG/goat): 8 mL x 1 bottle
    • Positive Control: 1.5 mL x 1 vial
    • Negative Control: 1.5 mL x 1 vial
    • Assay Diluent: 50 mL x 1 bottle
    • Wash Concentrate (20X): 50 mL x 1 bottle
    • Substrate Solution (TMB/H2O2): 20 mL x 1 bottle
    • Stop Solution: 20 mL x 1 bottle
  • Description:

    The anti-Type VII collagen ELISA Kit is an Enzyme Linked Immuno-Sorbent assay (ELISA) detection kit for anti-type VII collagen antibody in serum.

    For Research Use Only. Not for use in diagnostic procedures.

  • Product Type:
    ELISA Kit
  • Research Area:
    Autoimmune
  • Short Description:

    The anti-Type VII collagen ELISA Kit is an Enzyme Linked Immuno-Sorbent assay (ELISA) detection kit

    for anti-type VII collagen antibody in serum.

    For Research Use Only. Not for use in diagnostic procedures.

  • Size:
    48 wells
References
  1. Lapiere JC, Woodley DT, Parente MG, Iwasaki T, Wynm KC, Christiano AM, and Uitto J : Epitope mapping of type collagen. Identification of discrete peptide sequence recognized by sera patients with acquired epidermolysisbullosa. J Clin Invest 92; 1831-1839, 1993
  2. Chen M, Chan LS, Cai X, O'Tool EA, Sample JC, and Woodly DT: Development of an ELISA for rapid detection of anti-type collagen autoantibodies in epidermolysis bullosa acquisita. J Invest Dermatol 108; 68-72, 1997
  3. Woodley DT, Chang C, Saadat P, Ram R, Liu Z, and Chen M: Evidence that anti-typeⅦ collagen antibodies are pathogenic and responsible for the clinical, histological, and immunological feature of epidermolysis bullosa acquisita. J Invest dermatol 124; 958-964, 2005
  4. Chen M, Keene DR, Costa FK, Tahk SH, and Woodley DT: The carboxyl terminus of typeⅦ collagen mediates antiparallel dimer formation and constitutes a new antigenic epitope for epidermolysis bullosa acquisita autoantibodies. J Biol Chem 276; 21649-21655, 2001