MESACUP BP180 TEST (EU Only)

  • Applications
    • ELISA
  • Code # RG-7695EC-D
  • Size 48 wells
  • Price Call for Price
Specifications

Background

BP180 is thought to be the direct target of the autoantibody because of its location, and the autoantibodies against BP230 are thought to be secondarily produced. The antibodies against BP180 are thought to be pathogenic, because the rabbit antibody against mouse NC16a region of BP180 forms blister similar to BP when injected to neonatal mice (1). The main epitope of BP180 is located in the region close to cell membrane called NC16a and most of patient serum react with the recombinant NC16a protein (2). The MESACUP BP180 TEST is for measuring anti BP180 autoantibodies in patient serum specifically.

  • Application:
    ELISA
  • Components:
    <ol> <li><span>BP180 MICROWELL STRIPS: 48 wells (6 x 8 wells) coated with recombinant purified BP 180NC16a antigen, the breakaway strips packed in a strip holder and sealed in a foil envelope with desiccant, are stable at 2-8&deg;C&nbsp;until labeled expiration date.</span></li> <li><span>Calibrator 1 (0 U /mL): One vial containing 1.5 mL of Assay Diluent including 0.09% sodium azide. Stable at 2-8&deg;C&nbsp;until labeled expiration date.</span></li> <li><span>Calibrator 2 (100 U/mL): One vial containing 1.5 mL of anti BP180 antibody positive human serum with Assay Diluent including 0.09% sodium azide. Stable at 2-8&deg;C&nbsp;until labeled expiration date.</span></li> <li><span>Conjugate Reagent: </span><span>One vial containing 8 mL of horseradish peroxidase conjugated goat anti human IgG. Stable at 2-8&deg;C&nbsp;until labeled expiration date.</span></li> <li><span>Assay Diluent: </span><span>One vial containing 50 mL of PBS, Tween 20 and 0.09% sodium azide. Stable at 2-8&deg;C&nbsp;until labeled expiration date.</span></li> <li><span>Wash Concentrate (10x): </span><span>One vial containing 100 mL of PBS and Tween 20 as a 10x concentrate. Stable at 2-8&deg;C&nbsp;until labeled expiration date.</span></li> <li><span>Substrate: </span><span>One vial containing 20 mL of 3,3',5,5'-tetramethylbenzidine dihydrochloride/hydrogen peroxide (TMB/ H2O2). Stable at 2-8&deg;C&nbsp;until labeled expiration date.</span></li> <li><span>Stop Solution: </span><span>One vial containing 20 mL of 1.0N sulfuric acid. Stable at 2-8&deg;C&nbsp;until labeled expiration date.</span></li> </ol>
  • Description:

    INTENDED USE

    The MESACUP BP180 TEST is a semi-quantitative Enzyme-Linked Immunosorbent Assay (ELISA) for the detection of anti BP180 antibodies in human serum. The MESACUP BP180 TEST is intended for in vitro diagnostic use as an aid in the differential diagnosis of certain pemphigoid diseases.

    For sale in the EU only. Not for sale in the U.S. 

  • Product Type:
    ELISA Kit
  • Research Area:
    Autoimmune
  • Short Description:

    INTENDED USE

    The MESACUP BP180 TEST is a semi-quantitative enzyme-linked immunosorbent assay (ELISA) for the detection of anti BP180 antibodies in human serum. The MESACUP BP180 TEST is intended for in vitro diagnostic use as an aid in the differential diagnosis of certain pemphigoid diseases.

  • Size:
    48 wells
References
  1. Liu Z, et al.: A passive transfer model of the organ-specific autoimmune disease, bullous pemphigoid, using antibodies generated against the hemidesmosomal antigen, BP180. J Clin Invest 92:2480-2488, 1993.
  2. Matsumura K, et al.: The majority of bullous pemphigoid and herpes gestationis serum samples react
    with NC16a domain of the 180kD bullous pemphigoid antigen. Arch Dermatol Res 288:507-509, 1996.