LC3 Autophagy Reagents
LC3 has been studied extensively and is considered to be a hallmark for autophagy. LC3 is the mammalian homologue of yeast ATG8 (Autophagy related gene 8). LC3 has 3 different isoforms: LC3A, LC3B and LC3C. There are similarities with the 3 isoforms as well as differences. The protein structures of LC3 A, B and C have been determined and all three isoforms show a conserved region required for autophagosome formation. LC3B is considered to be a popular marker of autophagy. LC3A and LC3B show different expression patterns in rat tissue implicating there may be a functional difference.
Autophagy research is increasingly important in understanding processes in the body. In order to determine levels of autophagy, anti-LC3 antibodies can be used for detection of endogenous LC3-II by immunoblotting, immunoprecipitation, and immunofluorescence. MBL International provides high quality and highly cited monoclonal and polyclonal LC3 antibodies for use in various applications, including WB, ICC, IHC, IPP, and FCM. These LC3 autophagy reagents are capable of detecting various forms of LC3, LC3A, LC3B and LC3C.