Afamin/Wnt3a Condition Medium
Wnt signaling is known to be involved in early development, maintenance and regeneration of stem cells, and in cancer formation. Wnt3a has been revealed to be an essential niche component for maintaining the proliferation of Lgr5-positive stem cells in intestinal epithelial cells and is used for the production of various digestive organoids such as the small intestine, large intestine, stomach, pancreas and liver. Although Wnt3a has been conventionally used for the culture of gut organoids, it is a fat-soluble protein, therefore forms aggregates in serum-free medium and can not exert activity sufficiently. In 2016, Mihara et al. found that high Wnt3a activity can be maintained by forming a complex with Wnt3a by Afamin, which is one of the components of serum. Long-term culture of organoids becomes possible by using Afamin and Wnt3a complex for organoid culture. This new medium will result in optimal success for your organoid experiments.