Anti-Atg5 pAb (Polyclonal Antibody)

Atg5 Polyclonal Antibody.

Specifications:

Description

Polyclonal Antibody of 100 µl targeting Atg5 for WB.

Target: Atg5
Product Type: Antibody
Size: 100 µl
Application: WB
Research Area / Disease: Autophagy
Conjugate: Unlabeled
Antibody Type: Polyclonal
Formulation: 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Isotype: Affinity Purified Ig
Immunogen: C-terminal peptide of human Atg5
Host Species: Rabbit
Species Reactivity: Human, Mouse, Rat
Source: This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with the synthetic peptide corresponding to C-terminus of human Atg5.
Reactivity: This antibody reacts with Atg5-Atg12 complex (55 kDa) on Western blotting.
Gene ID Human:

9474

Gene ID Mouse:

11793

Storage Temperature: -20°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Citations

  1. Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med. 19, 1478-88 (2013),
  2. Manley S et al. Suppression of autophagic flux by bile acids in hepatocytes. Toxicol Sci. 137, 478-90 (2014),
  3. Myeku N, Figueiredo-Pereira ME. Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62. J Biol Chem. 286, 22426-40 (2011),
  4. Ni HM et al. Dissecting the dynamic turnover of GFP-LC3 in the autolysosome. Autophagy 7, 188-204 (2011),
  5. Ni HM et al. Critical role of FoxO3a in alcohol-induced autophagy and hepatotoxicity. Am J Pathol. 183, 1815-25 (2013)
  6. Li Y et al. Impaired Fasting-Induced Adaptive Lipid Droplet Biogenesis in Liver-Specific Atg5-Deficient Mouse Liver Is Mediated by Persistent Nuclear Factor-Like 2 Activation. Am J Pathol. 188, 1833-1846 (2018)
  7. Shen J et al. Role of Autophagy in Zinc Oxide Nanoparticles-Induced Apoptosis of Mouse LEYDIG Cells. Int J Mol Sci. 20, pii: E4042 (2019)
  8. Saladini S et al. Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells. Cells 8, E49 (2019)
  9. Wang, Jinglei, et al. “Tri-ortho-cresyl phosphate induces autophagy of mouse ovarian granulosa cells.” Reproduction 158.1 (2019): 61-69.
  10. Yang, Si, et al. “Tea polyphenols alleviate tri‐ortho‐cresyl phosphate‐induced autophagy of mouse ovarian granulosa cells.” Environmental toxicology 35.4 (2020): 478-486.
  11. Yang, Si, et al. “Tea polyphenols alleviate tri‐ortho‐cresyl phosphate‐induced autophagy of mouse ovarian granulosa cells.” Environmental toxicology 35.4 (2020): 478-486.
  12. Cocco, Stefania, et al. “Inhibition of autophagy by chloroquine prevents resistance to PI3K/AKT inhibitors and potentiates their antitumor effect in combination with paclitaxel in triple negative breast cancer models.” Journal of Translational Medicine 20.1 (2022): 290.
  13. Yang, Si, et al. “Role of autophagy in lysophosphatidylcholine-induced apoptosis of mouse ovarian granulosa cells.” International journal of molecular sciences 23.3 (2022): 1479.

References

  1. Hanada, T., et al., J. Biol. Chem. 282, 37298-37302 (2007)
  2. Pyo, J. O., et al., J. Biol. Chem. 280, 20722-20729 (2005)
  3. Mizushima, N., et al., J. Cell Biol. 152, 657-667 (2001)