Anti-Atg5 pAb

  • Applications
    • WB
  • Target Atg5
  • Host Species Rabbit
  • Species Reactivities Human, Mouse, Rat
  • Code # PM050
  • Size 100 μl
  • Price
    $289.63
Specifications

Background

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Autophagy has two ubiquitin-like conjugation systems, the Atg12 and LC3-II systems. In the Atg12 conjugation system, the Atg12-Atg5-Atg16L forms 800 kDa complex that elongates autophagic isolation membrane. After completion of the formation of the autophagosome, the Atg12-Atg5-Atg16L complex dissociates from the membrane.
  • Antibody Type:
    Polyclonal
  • Application:
    WB
  • Conjugate:
    Unlabeled
  • Description:

    Polyclonal Antibody of 100 µl targeting Atg5 for WB.

  • Formulation:
    100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Rabbit
  • Immunogen:
    C-terminal peptide of human Atg5
  • Isotype:
    Affinity Purified Ig
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with Atg5-Atg12 complex (55 kDa) on Western blotting.
  • Research Area:
    Autophagy
  • Short Description:

    Atg5 Polyclonal Antibody.

  • Size:
    100 μl
  • Species Reactivity:
    Human, Mouse, Rat
  • Storage Temperature:
    -20°C
  • Target:
    Atg5
Citations
  1. Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med. 19, 1478-88 (2013),
  2. Manley S et al. Suppression of autophagic flux by bile acids in hepatocytes. Toxicol Sci. 137, 478-90 (2014),
  3. Myeku N, Figueiredo-Pereira ME. Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62. J Biol Chem. 286, 22426-40 (2011),
  4. Ni HM et al. Dissecting the dynamic turnover of GFP-LC3 in the autolysosome. Autophagy 7, 188-204 (2011),
  5. Ni HM et al. Critical role of FoxO3a in alcohol-induced autophagy and hepatotoxicity. Am J Pathol. 183, 1815-25 (2013)
References
  1. Hanada, T., et al., J. Biol. Chem. 282, 37298-37302 (2007)
  2. Pyo, J. O., et al., J. Biol. Chem. 280, 20722-20729 (2005)
  3. Mizushima, N., et al., J. Cell Biol. 152, 657-667 (2001)