Anti-p62 (SQSTM1) (Human) pAb (Polyclonal Antibody)

p62 Polyclonal Antibody.

Specifications:

Description

This antibody is validated for multiple applications (WB, IHC, ICC and IP) and is widely cited, including in research papers with more than 200 citations (Waguri S, Komatsu M. Biochemical and morphological detection of inclusion bodies in autophagy-deficient mice. Methods Enzymol. 453, 181-96 (2009)). This is a polyclonal antibody of 100 ul that is raised in rabbit and is reactive with human, hamster, mouse, rat.

Target: p62
Product Type: Antibody
Size: 100 µl
Application: ICC, IHC, IP, WB
Research Area / Disease: Autophagy
Conjugate: Unlabeled
Antibody Type: Polyclonal
Formulation: 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Isotype: Affinity Purified Ig
Immunogen: Recombinant human p62 (120-440 a.a.)
Host Species: Rabbit
Species Reactivity: Hamster, Human, Mouse, Rat
Source: This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with the recombinant human p62 protein corresponding to amino acids 120-440.
Reactivity: This antibody reacts with p62 on Western blotting, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Gene ID Human:

8878

Gene ID Mouse:

18412

Gene ID Rat:

113894

Storage Temperature: -20°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Citations

  1. Ito T et al. Cold PSM, but not TRAIL, triggers autophagic cell death: A therapeutic advantage of PSM over TRAIL. Int J Oncol. 53, 503-514 (2018)
  2. Zhan R et al. Dapsone protects brain microvascular integrity from high-fat diet induced LDL oxidation. Cell Death Dis. 9, 683 (2018)
  3. Runwal, G., Stamatakou, E., Siddiqi, F. et al. LC3-positive structures are prominent in autophagy-deficient cells. Sci Rep 9, 10147 (2019)
  4. Adams O et al. A specific expression profile of LC3B and p62 is associated with nonresponse to neoadjuvant chemotherapy in esophageal adenocarcinomas. PLoS One 13, e0197610 (2018)
  5. Matsumoto G et al. N-Acyldopamine induces aggresome formation without proteasome inhibition and enhances protein aggregation via p62/SQSTM1 expression. Sci Rep. 8, 9585 (2018)
  6. Lin X et al. Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy. Cell Death Dis. 9, 768 (2018)
  7. Chen B et al. Comparative Study of Different Diets-Induced NAFLD Models of Zebrafish. Front Endocrinol 9, 366 (2018)
  8. Osaki Y et al. Shutdown of ER-associated degradation pathway rescues functions of mutant iduronate 2-sulfatase linked to mucopolysaccharidosis type II. Cell Death Dis. 9, 808(2018)
  9. Qiu J et al. Distinct subgroup of the Ras family member 3 (DIRAS3) expression impairs metastasis and induces autophagy of gastric cancer cells in mice. J Cancer Res Clin Oncol. 144, 1869-1886 (2018)
  10. Sun Y et al. Ischemic Postconditioning Alleviates Cerebral Ischemia-Reperfusion Injury Through Activating Autophagy During Early Reperfusion in Rats. Neurochem Res. 43, 1826-1840 (2018)
  11. Bak DH et al. Anti‑apoptotic effects of human placental hydrolysate against hepatocyte toxicity in vivo and in vitro. Int J Mol Med. 42, 2569-2583 (2018)
  12. Sil S et al. Cocaine Mediated Neuroinflammation: Role of Dysregulated Autophagy in Pericytes. Mol Neurobiol. (2018) In press.
  13. Liu X et al. Deacetylation of NAT10 by Sirt1 promotes the transition from rRNA biogenesis to autophagy upon energy stress. Nucleic Acids Res. 46, 9601-9616 (2018)
  14. Götzl JK et al. Early lysosomal maturation deficits in microglia triggers enhanced lysosomal activity in other brain cells of progranulin knockout mice. Mol Neurodegener. 13, 48 (2018)
  15. Jin GL et al. Koumine Decreases Astrocyte-Mediated Neuroinflammation and Enhances Autophagy, Contributing to Neuropathic Pain From Chronic Constriction Injury in Rats. Front Pharmacol. 9, 989 (2018)
  16. Gkirtzimanaki K et al. IFNα Impairs Autophagic Degradation of mtDNA Promoting Autoreactivity of SLE Monocytes in a STING-Dependent Fashion. Cell Rep. 25, 921-933.e5 (2018)
  17. Liu N et al. Lactate inhibits ATP6V0d2 expression in tumor-associated macrophages to promote HIF-2 Α -mediated tumor progression. J Clin Invest. 129 631-646 (2019)
  18. Sciarrillo R et al. Splicing modulation as novel therapeutic strategy against diffuse malignant peritoneal mesothelioma. EBioMedicine 39, 215-225 (2019)
  19. Aki T et al. Formation of high molecular weight p62 by CORM-3. PLoS One. 14, e0210474 (2019)
  20. Hsu YH et al. Traffic-related particulate matter exposure induces nephrotoxicity in vitro and in vivo. Free Radic Biol Med. 135, 235-244 (2019)
  21. Hoffmann AC et al. Extracellular aggregated alpha synuclein primarily triggers lysosomal dysfunction in neural cells prevented by trehalose. Sci Rep. 9, 544 (2019)rnrn
  22. Scrivo A et al. Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production. Nat Commun. 10, 780 (2019
  23. Homma T, Fujii J. An SOD1 deficiency aggravates proteasome inhibitor bortezomib-induced testicular damage in mice. Biochim Biophys Acta Gen Subj. 1863, 1108-1115 (2019)
  24. Ohashi T et al. Conophylline inhibits high fat diet-induced non-alcoholic fatty liver disease in mice. PLoS One. 14, e0210068 (2019)rnrn
  25. Yang Y et al. Cytoplasmic DAXX drives SQSTM1/p62 phase condensation to activate Nrf2-mediated stress response. Nat Commun. 10, 3759 (2019)
  26. Morishita Het al. A critical role of VMP1 in lipoprotein secretion. Elife.. 8, e48834 (2019)
  27. Miao X et al. Serum SQSTM1 is a potential predictor for chemotherapeutic efficacy against non-small cell lung . Int J Clin Exp Med. 11, 5811-5819 (2018)

References

  1. Komatsu, M., et al., Cell 131, 1149-1163 (2007)
  2. Moscat, J., et al., Mol. Cell 23, 631-640 (2006)
  3. Seibenhener, M. L., et al., Mol. Cell Biol. 24, 8055-8068 (2004)