Anti-Atg16L (Human) pAb

  • Applications
    • ICC
    • IP
    • WB
  • Target Atg16L
  • Host Species Rabbit
  • Species Reactivities Hamster, Human, Mouse, Rat
  • Code # PM040
  • Size 100 µg
  • Price
    $312.80
Specifications

Alternative Names

ATG16L1, autophagy related 16-like 1 (S. cerevisiae), IBD10, WDR30, APG16L, ATG16A, Apg16l

Background

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. LC3, GABARAP (γ-aminobutyric-acid-type-A-receptor-associated protein), and GATE-16 (Golgi-associated ATPase enhancer of 16 kDa) have been identified as a homologue of yeast Atg8. These homologues have been characterized as modifiers in reactions mediated by hAtg7 (an E1-like enzyme) and hAtg3 (an E2-like enzyme) as in yeast Atg8 lipidation. These homologues also generate form II, which are recovered in membrane fractions. Generation of the form II correlates with autophagosome association. These results suggest that all mammalian Atg8 homologues receive a common modification to associate with autophagosomal membrane as the form II.
  • Antibody Type:
    Polyclonal
  • Application:
    ICC, IP, WB
  • Conjugate:
    Unlabeled
  • Description:

    Polyclonal Antibody of 100 µg targeting Atg16L for ICC, IP, WB.

  • Formulation:
    100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Gene ID (Rat):
  • Host Species:
    Rabbit
  • Immunogen:
    Recombinant human ATG16L1 TV2 (85-588 a.a.)
  • Isotype:
    Affinity Purified Ig
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with Atg16L onWestern blotting, Immunoprecipitation andImmunocytochemistry.
  • Research Area:
    Autophagy
  • Short Description:

    Atg16L Polyclonal Antibody.

  • Size:
    100 µg
  • Species Reactivity:
    Hamster, Human, Mouse, Rat
  • Storage Temperature:
    -20°C
  • Target:
    Atg16L
Citations
  1. Matsunaga K et al. Autophagy requires endoplasmic reticulum targeting of the PI3-kinase complex via Atg14L. J Cell Biol. 190, 511-21 (2010),
  2. Moreau K et al. Arf6 promotes autophagosome formation via effects on phosphatidylinositol 4,5-bisphosphate and phospholipase D. J Cell Biol. 196, 483-96 (2012),
  3. Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014),
  4. Takahashi Y et al. Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy. Autophagy 7, 61-73 (2011),
  5. Young MM et al. Autophagosomal membrane serves as platform for intracellular death-inducing signaling complex (iDISC)-mediated caspase-8 activation and apoptosis. J Biol Chem. 287, 12455-68 (2012)
References
  1. Murthy, A., et al., Nature 506, 456-462 (2014) [IP]
  2. Young, M. M., et al., J.Biol.Chem. 287, 12455-12468 (2012)[IC]
  3. Moreau, K., et al., J. Cell Biol. 196, 483-496 (2012) [IC]
  4. Takahashi, Y., et al., Autophagy 7, 61-73 (2011) [IC]
  5. Matsunaga, K., et al., J. Cell Biol. 190, 511-521 (2010) [IC]
  6. Matsushita, M., et al., J. Biol. Chem. 282, 6763-6772 (2007)