Anti-LC3 pAb (Polyclonal Antibody)

LC3 Polyclonal Antibody.



Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect forms of LC3 (MAP1LC3A, B, C).


This LC3 antibody is validated for multiple applications (WB, IHC, ICC and IP) and isoform detection. PM036 has more than 1,000 citations where it used to generate data showing autophagy in research papers.  (Saitoh T et al. Loss of the autophagy protein Atg16L1 enhances endotoxin-induced IL-1beta production. Nature 456, 264-8 (2008)). This is a polyclonal antibody that is raised in rabbit and is reactive with human, hamster, mouse, rat.


Target: LC3
Product Type: Antibody
Size: 100 µl
Application: FCM, ICC, IHC, IP, WB
Research Area / Disease: Autophagy
Conjugate: Unlabeled
Antibody Type: Polyclonal
Formulation: 100 L volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Isotype: IgG
Immunogen: Recombinant human LC3 (MAP1LC3B :1-120 a.a.)
Host Species: Rabbit
Species Reactivity: Hamster, Human, Mouse, Rat
Source: This antibody was purified from rabbit serum using protein A agarose. The rabbit was immunized with the recombinant human LC3 [MAP1LC3B (1-120 aa)].
Reactivity: This antibody reacts with LC3 (MAP1LC3A, B, C) on Western blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow cytometry. It does not react with GABARAP and GATE-16.
Gene ID Human:

81631, 84557, 440730

Gene ID Mouse:

67443,  66734

Gene ID Rat:


Storage Temperature: -20°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.


See even more publications

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Pan JA, et al., Mol Cell Biol, 31, 3158 (2011) : WB

Huang Y, et al., Autophagy, 7, 1132 (2011) : WB

Hasui K, et al., Acta Histochem Cytochem, 44, 119 (2011) : IHC-P

Miranda S, et al., J Cell Physiol, 227, 2352 (2012) : WB

Otomo T, et al., J Biol Chem, 286, 35283 (2011) : WB

Yan J, et al., Anticancer Drugs, 23, 22 (2012) : IC, WB

Takaesu G, et al., J Biochem, 151, 157 (2012) : WB

Taguwa S, et al., J Virol, 85, 13185 (2011) : WB, IC

Xu T, et al., Autophagy, 7, 1316 (2011) : WB

Rasmussen SB, et al., J Immunol, 187, 5268 (2011) : WB, IC

Wu SY, et al., Neoplasia, 13, 1171 (2011) : WB

Starr T, et al., Cell Host Microbe, 11, 33 (2012) : WB

Kaini RR, et al., Prostate, 72, 1412 (2012) : WB

Yu X, et al., J Mol Neurosci, 47, 219 (2012) : WB

Niizuma S, et al., Life Sci, 90, 619 (2012) : WB

Seillier M, et al., Cell Death Differ, 19, 1525 (2012) : IC

Kaminskyy V, et al., Autophagy, 8, 1032 (2012) : WB, IC

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