Anti-LC3 pAb (Polyclonal Antibody)

LC3 Polyclonal Antibody.

Specifications:

Description

This LC3 antibody is validated for multiple applications (WB, IHC, ICC and IP) and has over 85 citations, including in research papers with more than 1,000 citations (Saitoh T et al. Loss of the autophagy protein Atg16L1 enhances endotoxin-induced IL-1beta production. Nature 456, 264-8 (2008)). This is a polyclonal antibody of 100 ul that is raised in rabbit and is reactive with human, hamster, mouse, rat.

 

Target: LC3
Product Type: Antibody
Size: 100 µl
Application: FCM, ICC, IHC, IP, WB
Research Area / Disease: Autophagy
Conjugate: Unlabeled
Antibody Type: Polyclonal
Isotype: IgG
Immunogen: Recombinant human LC3 (MAP1LC3B :1-120 a.a.)
Host Species: Rabbit
Species Reactivity: Hamster, Human, Mouse, Rat
Source: This antibody was purified from rabbit serum using protein A agarose. The rabbit was immunized with the recombinant human LC3 [MAP1LC3B (1-120 aa)].
Reactivity: This antibody reacts with LC3 (MAP1LC3A, B, C) on Western blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow cytometry. It does not react with GABARAP and GATE-16.
Gene ID Human:

81631, 84557, 440730

Gene ID Mouse:

67443,  66734

Gene ID Rat:

64862362245

Storage Temperature: -20°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Citations

  1. Hasui K et al. Enhanced Autophagy and Reduced Expression of Cathepsin D Are Related to Autophagic Cell Death in Epstein-Barr Virus-Associated Nasal Natural Killer/T-Cell Lymphomas: An Immunohistochemical Analysis of Beclin-1, LC3, Mitochondria (AE-1), and Cathepsin D in Nasopharyngeal Lymphomas. Acta Histochem Cytochem. 44, 119-31 (2011),
  2. Huang Y et al. PML-RARα enhances constitutive autophagic activity through inhibiting the Akt/mTOR pathway. Autophagy 7, 1132-44 (2011),
  3. Kaini RR et al. Autophagy regulates lipolysis and cell survival through lipid droplet degradation in androgen-sensitive prostate cancer cells. Prostate 72, 1412-22 (2012),
  4. Kaminskyy VO et al. Suppression of basal autophagy reduces lung cancer cell proliferation and enhances caspase-dependent and -independent apoptosis by stimulating ROS formation. Autophagy 8, 1032-44 (2012),
  5. Katona I et al. Distinct pathogenic processes between Fig4-deficient motor and sensory neurons. Eur J Neurosci. 33, 1401-10 (2011),
  6. Lee EJ and Tournier C. The requirement of uncoordinated 51-like kinase 1 (ULK1) and ULK2 in the regulation of autophagy. Autophagy. 7, 689-95 (2011),
  7. Miranda S et al. Beneficial effects of fenofibrate in retinal pigment epithelium by the modulation of stress and survival signaling under diabetic conditions. J Cell Physiol. 227, 2352-62 (2012),
  8. Niizuma S et al. Effect of persistent activation of phosphoinositide 3-kinase on heart. Life Sci. 90, 619-28 (2012),
  9. Otomo T et al. Lysosomal storage causes cellular dysfunction in mucolipidosis II skin fibroblasts. J Biol Chem. 286, 35283-90 (2011),
  10. Pan JA et al. Inhibition of protein degradation induces apoptosis through a microtubule-associated protein 1 light chain 3-mediated activation of caspase-8 at intracellular membranes. Mol Cell Biol. 31, 3158-70 (2011),
  11. Rasmussen SB et al. Activation of autophagy by α-herpesviruses in myeloid cells is mediated by cytoplasmic viral DNA through a mechanism dependent on stimulator of IFN genes. J Immunol. 187, 5268-76 (2011),
  12. Seillier M et al. TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death. Cell Death Differ. 19, 1525-35 (2012),
  13. Starr T et al. Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle. Cell Host Microbe. 11, 33-45 (2012),
  14. Taguwa S et al. Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus. J Virol. 85, 13185-94 (2011),
  15. Takaesu G et al. TGFβ-activated kinase 1 (TAK1)-binding proteins (TAB) 2 and 3 negatively regulate autophagy. J Biochem. 151, 157-66 (2012),
  16. Wu SY et al. Ras-related tumorigenesis is suppressed by BNIP3-mediated autophagy through inhibition of cell proliferation. Neoplasia 13, 1171-82 (2011),
  17. Wu YN et al. The selective growth inhibition of oral cancer by iron core-gold shell nanoparticles through mitochondria-mediated autophagy. Biomaterials. 32, 4565-73 (2011),
  18. Xu T et al. Modulation of autophagic activity by extracellular pH. Autophagy 7, 1316-22 (2011),
  19. Yan J et al. Methyl-β-cyclodextrin induces programmed cell death in chronic myeloid leukemia cells and, combined with imatinib, produces a synergistic downregulation of ERK/SPK1 signaling. Anticancer Drugs. 23, 22-31 (2012),
  20. Yu X et al. Differential degradation of full-length and cleaved ataxin-7 fragments in a novel stable inducible SCA7 model. J Mol Neurosci. 47, 219-33 (2012)
  21. Wan G, et al., J Biol Chem, 283, 21540 (2008) : WB
  22. Ohne Y, et al., J Biol Chem, 283, 31861 (2008) : WB
  23. Saitoh T, et al., Nature, 456, 264 (2008) : WB
  24. Sasnauskiene A, et al., Apoptosis, 14, 276 (2009) : WB, IC
  25. Kimura S, et al., Methods Enzymol, 452, 1 (2009) : WB
  26. Chung JW, et al., PLoS Pathog, 5, e1000561 (2009) : IC
  27. Yoshikawa Y, et al., Nat Cell Biol, 11, 1233 (2009) : WB
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  30. Blanchet FP, et al., Immunity, 32, 654 (2010) : WB
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  34. Tian F, et al., Autophagy, 6, 1107 (2010) : WB, IHC
  35. Tabata K, et al., Mol Biol Cell, 21, 4162 (2010) : WB
  36. Kaminskyy V, et al., Autophagy, 7, 83 (2011) : WB, IC, FCM
  37. Kobayashi H, et al., Mol Genet Metab, 102, 170 (2011) : IHC-P
  38. Chen SY, et al., Autophagy, 7, 217 (2011) : WB
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  44. Salazar M, et al., Methods Enzymol, 489, 297 (2011) : WB, IC
  45. Ishii Y, et al., Clin Cancer Res, 17, 2292 (2011) : WB
  46. Katona I, et al., Eur J Neurosci, 33, 1401 (2011) : IHC
  47. Wu YN, et al., Biomaterials, 32, 4565 (2011) : IC
  48. Lee EJ, et al., Autophagy, 7, 689 (2011) : WB
  49. Pan JA, et al., Mol Cell Biol, 31, 3158 (2011) : WB
  50. Huang Y, et al., Autophagy, 7, 1132 (2011) : WB
  51. Hasui K, et al., Acta Histochem Cytochem, 44, 119 (2011) : IHC-P
  52. Miranda S, et al., J Cell Physiol, 227, 2352 (2012) : WB
  53. Otomo T, et al., J Biol Chem, 286, 35283 (2011) : WB
  54. Yan J, et al., Anticancer Drugs, 23, 22 (2012) : IC, WB
  55. Takaesu G, et al., J Biochem, 151, 157 (2012) : WB
  56. Taguwa S, et al., J Virol, 85, 13185 (2011) : WB, IC
  57. Xu T, et al., Autophagy, 7, 1316 (2011) : WB
  58. Rasmussen SB, et al., J Immunol, 187, 5268 (2011) : WB, IC
  59. Wu SY, et al., Neoplasia, 13, 1171 (2011) : WB
  60. Starr T, et al., Cell Host Microbe, 11, 33 (2012) : WB
  61. Kaini RR, et al., Prostate, 72, 1412 (2012) : WB
  62. Yu X, et al., J Mol Neurosci, 47, 219 (2012) : WB
  63. Niizuma S, et al., Life Sci, 90, 619 (2012) : WB
  64. Seillier M, et al., Cell Death Differ, 19, 1525 (2012) : IC
  65. Kaminskyy V, et al., Autophagy, 8, 1032 (2012) : WB, IC
  66. Gafni J, et al., J Neurosci, 32, 7454 (2012) : WB, IHC-P
  67. Sugimoto M, et al., G3 (Bethesda), 2, 1077 (2012) : WB
  68. Kang YA, et al., Mol Cell Biol, 32, 226 (2012) : WB
  69. Bak DH, Zhang E, Yi MH, et al. High ω3-polyunsaturated fatty acids in fat-1 mice prevent streptozotocin-induced Purkinje cell degeneration through BDNF-mediated autophagy. Sci Rep. 2015;5:15465.
  70. Campbell-valois FX, Sachse M, Sansonetti PJ, Parsot C. Escape of Actively Secreting Shigella flexneri from ATG8/LC3-Positive Vacuoles Formed during Cell-To-Cell Spread Is Facilitated by IcsB and VirA. MBio. 2015;6(3):e02567-14.
  71. Katagiri N, Kuroda T, Kishimoto H, Hayashi Y, Kumazawa T, Kimura K. The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. Sci Rep. 2015;5:8903.
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  73. Shao W, Espenshade PJ. Sterol regulatory element-binding protein (SREBP) cleavage regulates Golgi-to-endoplasmic reticulum recycling of SREBP cleavage-activating protein (SCAP). J Biol Chem. 2014;289(11):7547-57.
  74. Valapala M, Wilson C, Hose S, et al. Lysosomal-mediated waste clearance in retinal pigment epithelial cells is regulated by CRYBA1/βA3/A1-crystallin via V-ATPase-MTORC1 signaling. Autophagy. 2014;10(3):480-96.
  75. Zhai Z, Wu F, Dong F, et al. Human autophagy gene ATG16L1 is post-transcriptionally regulated by MIR142-3p. Autophagy. 2014;10(3):468-79.
  76. Kim C, Bergelson JM. Echovirus 7 entry into polarized caco-2 intestinal epithelial cells involves core components of the autophagy machinery. J Virol. 2014;88(1):434-43.
  77. Zheng Y, Kielian M. Imaging of the alphavirus capsid protein during virus replication. J Virol. 2013;87(17):9579-89.
  78. Ciarcia R, Damiano S, Montagnaro S, et al. Combined effects of PI3K and SRC kinase inhibitors with imatinib on intracellular calcium levels, autophagy, and apoptosis in CML-PBL cells. Cell Cycle. 2013;12(17):2839-48.
  79. Russell RC, Tian Y, Yuan H, et al. ULK1 induces autophagy by phosphorylating Beclin-1 and activating VPS34 lipid kinase. Nat Cell Biol. 2013;15(7):741-50.
  80. Wang BS, Liu YZ, Yang Y, et al. Autophagy negatively regulates cancer cell proliferation via selectively targeting VPRBP. Clin Sci. 2013;124(3):203-14.
  81. Zhang X, Garbett K, Veeraraghavalu K, et al. A role for presenilins in autophagy revisited: normal acidification of lysosomes in cells lacking PSEN1 and PSEN2. J Neurosci. 2012;32(25):8633-48.
  82. Yu X, Ajayi A, Boga NR, Ström AL. Differential degradation of full-length and cleaved ataxin-7 fragments in a novel stable inducible SCA7 model. J Mol Neurosci. 2012;47(2):219-33.
  83. Otomo T, Higaki K, Nanba E, Ozono K, Sakai N. Lysosomal storage causes cellular dysfunction in mucolipidosis II skin fibroblasts. J Biol Chem. 2011;286(40):35283-90.
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  85. Ishii Y, Papa L, Bahadur U, et al. Bortezomib enhances the efficacy of fulvestrant by amplifying the aggregation of the estrogen receptor, which leads to a proapoptotic unfolded protein response. Clin Cancer Res. 2011;17(8):2292-300.
  86. Arsov I, Adebayo A, Kucerova-levisohn M, et al. A role for autophagic protein beclin 1 early in lymphocyte development. J Immunol. 2011;186(4):2201-9.
  87. Blanchet FP, Moris A, Nikolic DS, et al. Human immunodeficiency virus-1 inhibition of immunoamphisomes in dendritic cells impairs early innate and adaptive immune responses. Immunity. 2010;32(5):654-69.
  88. Williams T, Forsberg LJ, Viollet B, Brenman JE. Basal autophagy induction without AMP-activated protein kinase under low glucose conditions. Autophagy. 2009;5(8):1155-65.
  89. Härtlova A et al. LRRK2 is a negative regulator of Mycobacterium tuberculosis phagosome maturation in macrophages. EMBO J. 37, e98694 (2018)
  90. Leidal, A.M., Huang, H.H., Marsh, T. et al. The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles. Nat Cell Biol 22, 187–199 (2020).
  91. Alvarez-Garcia O et al. FOXO are required for intervertebral disk homeostasis during aging and their deficiency promotes disk degeneration. Aging Cell. 17, e12800 (2018)
  92. Phadwal K et al. Spermine increases acetylation of tubulins and facilitates autophagic degradation of prion aggregates. Sci Rep. 8, 10004 (2018)
  93. Chen B et al. Comparative Study of Different Diets-Induced NAFLD Models of Zebrafish. Front Endocrinol 9, 366 (2018)
  94. Sun Y et al. Ischemic Postconditioning Alleviates Cerebral Ischemia-Reperfusion Injury Through Activating Autophagy During Early Reperfusion in Rats. Neurochem Res. 43, 1826-1840 (2018)
  95. Skah S et al. cAMP-mediated autophagy inhibits DNA damage-induced death of leukemia cells independent of p53. Oncotarget 9, 30434–30449 (2018)
  96. Bak DH et al. Anti‑apoptotic effects of human placental hydrolysate against hepatocyte toxicity in vivo and in vitro. Int J Mol Med. 42, 2569-2583 (2018)
  97. Mohamud Y et al. CALCOCO2/NDP52 and SQSTM1/p62 differentially regulate coxsackievirus B3 propagation. Cell Death Differ. (2018) In pre
  98. Hagio-Izaki K et al. Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus. BMC Cell Biol. 19, 18 (2018)
  99. Ashraf NS et al. Citalopram reduces aggregation of ATXN3 in a YAC transgenic mouse model of Machado-Joseph disease. Mol Neurobiol. (2018) In press.
  100. Jiao YN et al. Marsdenia tenacissima extract induces apoptosis and suppresses autophagy through ERK activation in lung cancer cells. Cancer Cell Int. 18, 149 (2018)
  101. Kim JK et al. GABAergic signaling linked to autophagy enhances host protection against intracellular bacterial infections. Nat Commun. 9, 4184 (2018)
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References

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