Anti-LC3 (Rat) pAb

  • Applications
    • WB
  • Target LC3
  • Host Species Rabbit
  • Species Reactivities Hamster, Human, Mouse, Rat
  • Code # PD014
  • Size 100 μl
  • Price

Alternative Names

microtubule-associated protein 1 light chain 3 alpha, MAP1BLC3, MAP1ALC3, LC3, LC3A, ATG8E


Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3.
  • Antibody Type:
  • Application:
  • Conjugate:
  • Description:

    Polyclonal Antibody of 100 µl targeting LC3 for WB.

  • Formulation:
    100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
  • Immunogen:
    Full-length recombinant rat LC3
  • Isotype:
  • Product Type:
  • Reactivity:
    This antibody reacts with LC3-I (18kDa) and LC3-II (16 kDa) on Western blotting.
  • Research Area:
  • Short Description:

    LC3 Polyclonal Antibody.

  • Size:
    100 μl
  • Species Reactivity:
    Hamster, Human, Mouse, Rat
  • Storage Temperature:
  • Target:
  1. Brown JE et al. Essential role of the redox-sensitive kinase p66shc in determining energetic and oxidative status and cell fate in neuronal preconditioning. J Neurosci. 30, 5242-52 (2010),
  2. Cui D et al. Propofol prevents autophagic cell death following oxygen and glucose deprivation in PC12 cells and cerebral ischemia-reperfusion injury in rats. PLoS One 7, e35324 (2012),
  3. Hariharan N et al. Oxidative stress stimulates autophagic flux during ischemia/reperfusion. Antioxid Redox Signal. 14, 2179-90 (2011),
  4. Hou W et al. Autophagic degradation of active caspase-8: a crosstalk mechanism between autophagy and apoptosis. Autophagy 6, 891-900 (2010),
  5. Jaeger PA et al. Regulation of amyloid precursor protein processing by the Beclin 1 complex. PLoS One 5, e11102 (2010),
  6. Kim JH et al. Involvement of mitophagy in oncogenic K-Ras-induced transformation: overcoming a cellular energy deficit from glucose deficiency. Autophagy 7, 1187-98 (2011),
  7. Kurz T et al. Cell sensitivity to oxidative stress is influenced by ferritin autophagy. Free Radic Biol Med. 50, 1647-58 (2011),
  8. Maycotte P et al. Apoptosis and autophagy in rat cerebellar granule neuron death: Role of reactive oxygen species. J Neurosci Res. 88, 73-85 (2010),
  9. O'Donovan TR et al. Induction of autophagy by drug-resistant esophageal cancer cells promotes their survival and recovery following treatment with chemotherapeutics. Autophagy 7, 509-24 (2011),
  10. Otomo T et al. Inhibition of autophagosome formation restores mitochondrial function in mucolipidosis II and III skin fibroblasts. Mol Genet Metab. 98, 393-9 (2009),
  11. Rouschop KM et al. Autophagy is required during cycling hypoxia to lower production of reactive oxygen species. Radiother Oncol. 92, 411-6 (2009),
  12. Rouschop KM et al. The unfolded protein response protects human tumor cells during hypoxia through regulation of the autophagy genes MAP1LC3B and ATG5. J Clin Invest. 120, 127-41 (2010),
  13. Son YO et al. Cadmium induces autophagy through ROS-dependent activation of the LKB1-AMPK signaling in skin epidermal cells. Toxicol Appl Pharmacol. 255, 287-96 (2011),
  14. Takamura A et al. Enhanced autophagy and mitochondrial aberrations in murine G(M1)-gangliosidosis. Biochem Biophys Res Commun. 367, 616-22 (2008),
  15. Vazquez-Martin A et al. Autophagy facilitates the development of breast cancer resistance to the anti-HER2 monoclonal antibody trastuzumab. PLoS One. 4, e6251 (2009),
  16. Vega-Naredo I et al. Melatonin modulates autophagy through a redox-mediated action in female Syrian hamster Harderian gland controlling cell types and gland activity. J Pineal Res. 52, 80-92 (2012),
  17. Vega-Naredo I et al. Sexual dimorphism of autophagy in Syrian hamster Harderian gland culminates in a holocrine secretion in female glands. Autophagy 5, 1004-17 (2009),
  18. Vega-Naredo I, Coto-Montes A. Physiological autophagy in the Syrian hamster Harderian gland. Methods Enzymol. 452, 457-76 (2009),
  19. Wang J et al. Possible role for external environmental stimuli in nasopharyngeal NK/T-cell lymphomas in the northeast of China with EBV infection-related autophagic cell death: a pathoepidemiological analysis. J Clin Exp Hematop. 49, 97-108 (2009),
  20. Wong HK et al. Blocking acid-sensing ion channel 1 alleviates Huntington's disease pathology via an ubiquitin-proteasome system-dependent mechanism. Hum Mol Genet. 17, 3223-35 (2008)
  21. Kabeya, Y., et al., J. Cell Sci. 117, 2805-2812 (2004)
  22. Mizushima, N., et al., Mol. Biol. Sci. 15, 1101-1111 (2004)
  23. Mizushima, N., et al., J. Cell Biol. 152, 657-667 (2001)
  24. Kabeya, Y., et al., EMBO J. 19, 1720-5728 (2000)