Anti-Bromodeoxyuridine mAb-PE

  • Applications
    • FCM
  • Target BrdU
  • Host Species Mouse
  • Species Reactivities Human
  • Code # MI-11-5
  • Size 1 mL (50 Tests)
  • Price
    $284.06
Specifications

Alternative Names

BrdU, 5-Bromo-2-Deoxyuridine

Background

BrdU (5-Bromo-2-Deoxyuridine) is a derivative of uridine that can substitute for thymidine during DNA synthesis. The detection of BrdU incorporation into DNA is a common method to quantify newly synthesized DNA and to identify cells in the S-phase of the cell cycle. BrdU incorporation is frequently used in proliferation assays to study DNA repair, sister chromatid exchange, and the cytokinetics of normal and neoplastic cells.
  • Antibody Type:
    Monoclonal
  • Application:
    FCM
  • Clone Number:
    2B1
  • Conjugate:
    PE
  • Description:

    Monoclonal Antibody conjugated to PE of 1 ml (50 tests) targeting BrdU for FCM.

  • Formulation:
    50 tests in 1mL volume of PBS containing 1% BSA and 0.09% NaN3.
  • Host Species:
    Mouse
  • Immunogen:
    5-Iodouridine-Ovalbumin
  • Isotype:
    IgG1
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with bromodeoxyuridine incorporated in the nuclei in Raji cells on Flow cytometry and it reacts with iododeoxyuridine, iodouridine and bromouridine, but does not react with thymidine on Flow cytometry.
  • Research Area:
    Cancer
  • Short Description:

    BrdU Monoclonal Antibody.

  • Size:
    1 mL (50 Tests)
  • Species Reactivity:
    Human
  • Storage Temperature:
    4°C
  • Target:
    BrdU
References
  1. Tani, H., et al., PLoS One 8, e55684 (2013) [RNA-IP]
  2. Tani, H., et al., RNA Biol. 9, 1370-1379 (2012) [RNA-IP]
  3. Yamazaki, S., et al.,EMBO J. 31, 3667-3677 (2012)[IC, DNA-IP]
  4. Sugimura, K., et al., J. Cell Biol. 183, 1203-1212 (2008) [IC]
  5. D’Ambrosio,C., et al., Genes Dev. 22, 2215-2227 (2008)[DNA-IP]
  6. Bermejo, R., et al. Genes Dev. 21, 1921-1936 (2007) [DNA-IP]
  7. Hirai, S., et al., J. Neurosci. 26, 11992-12002 (2006) [IHC]
  8. Yamashita, N., et al., J. Neurosci. 26, 13357-13362 (2006) [IHC]
  9. Yeom, S. Y., et al., Mol. Cell. Biol. 26, 4553-4563 (2006) [IHC]
  10. Imai, F., et al., Development 133, 1735-1744 (2006) [IHC]
  11. Itakura, E., et al., Mol. Biol. Cell 16, 5551-5562 (2005) [IF]
  12. Katou, Y., et al., Nature 424, 1078-1083 (2003) [DNA-IP]
  13. Ito, S., et al., J. Gen. Virol. 83, 2377-2383 (2002) [IC]
  14. Liu, Q., et al., Mol. Cell Biol. 19, 6229-6239 (1999) [FCM]
  15. Nakajima, T., et al., PNAS 95, 10590-10595 (1998) [FCM]
  16. Gonchoroff, N. J., et al., J. Immunol. Meth. 93, 97-101 (1986)
  17. Gonchoroff, N. J., et al., Cytometry 6, 506-512 (1985)
  18. Gratzner, H. G., et al., Cytometry 1, 385-389 (1981)