QuickSwitch™ Custom Tetramer Kits

MBL International offers QuickSwitch™ Custom Tetramer kit for you to create your own custom tetramer for human or mouse Class I MHC or for human Class II MHC Tetramers.  In one day, you can complete peptide-MHC exchange AND know the quantification of your exchange-  using only ONE kit.  Special/complicated/difficult to use technology such as UV cleavage is not used with this kit, making it easy to use for every lab.  This kit is supplied with a tetramer bound with an irrelevant peptide.  After adding the supplied peptide exchange factor and your peptide of interest, peptide exchange will occur if there is binding affinity of the peptide with the MHC molecule.  By using this QUANT kit, you can quantify the amount of exchange that occurred, thereby gathering binding affinity information for each MHC/peptide complex you are investigating.  This kit can be used to create custom tetramers, or for screening peptides, all in the same day.  QuickSwitch™ is a system that allows discrimination of MHC binding and non-binding peptides.  Peptide-MHC binding information/affinity is particularly essential for the screening of immunogenic peptides from infectious agents or cancer neoantigens.  Tetramers resulting from peptide exchange with selected peptides can then be used for immune monitoring.  Functional screening of peptides for MHC Class I or MHC Class II binding is essential for vaccine design and immune monitoring.  Start with your in-silico results, use QuickSwitch kit to discover peptide-MHC binding affinities, and end with your own specific T cell detection reagent!  Contact us for additional information, help with your set up, or to make a bulk purchase request!

Class I

Class II