QuickSwitch™ Custom Tetramer Kits
MBL International offers QuickSwitch™ kit for you to create your own custom tetramer. This kit is supplied with a tetramer bound with an irrelevant peptide. After adding a peptide exchange factor and your peptide of interest, peptide exchange will occur if there is binding affinity of the peptide with the MHC molecule. By using the QUANT kit, you can quantify the amount of exchange that occurred, thereby gathering binding affinity information for the MHC/peptide complex. This kit can be used to create custom tetramers, or for screening peptides, all in one day. QuickSwitch™ is a 90-min-assay system that allows discrimination of MHC binding from non-binding peptides.
This is particularly essential for the screening of immunogenic peptides from infectious agents or cancer neoantigens. Tetramers resulting from peptide exchange with selected peptides can then be used for immune monitoring. Functional screening of peptides for MHC class I binding is essential for vaccine design and immune monitoring.