MHC Tetramers- Non-Classical
MBL International offers tetramers for the following non-classical MHC targets: HLA-E, Qa-1b, and MR1. Human leukocyte antigen E (HLA-E) is a non-classical class I molecule recognized by natural killer (NK) cells and CD8+ T cells. HLA-E binds to TCR expressed on CD8+ T cells, resulting in the T cell activation. The HLA-E tetramer is comprised of human class I HLA-E*01:03 and epitope peptide derived from the HLA-A leader, and it can detect HLA-E*01:03-restricted HLA-A leader3-11-specific NK cells and CD8+ T cells by flow cytometry. The non-classical class I mouse homolog of HLA-E tetramer, Qa-1b tetramer, is now available.
MHC class I-related protein (MR1) is classified as a non-classical class I molecule and has the ability to activate mucosal-associated invariant T cells (MAIT cells) by presenting vitamin B metabolites to them. Human MR1 Tetramer recognizes human MAIT cells that binds to specifically to the MR1/MR1-ligand complex.