Anti-NFIL3 (E4BP4) chimeric mAb (Monoclonal Antibody)

Anti-NFIL3 (E4BP4) chimeric mAb



NFIL3, or E4BP4, is a transcription factor that controls sleep and circadian rhythms throughout the cranial nerve, endocrine, and metabolic systems.The circadian clock is a 24-hour endogenous oscillator, which cycles via transcriptional/translational feedback loops of clock genes and their products in individual cells. CLOCK and BMAL1 are positive factors in the loop, and their complex binds to E-box elements of PERs and CRYs. PER and CRY bind the CLOCK-BMAL1 complex, suppressing E-box-dependent transactivation and forming a molecular clock via this negative feedback mechanism.D-box and REV-ERB/ROR-binding elements also form a regulatory network of clock-controlled genes (CCGs). D-box-dependent transactivation is activated by DBP and repressed by NFIL3/E4BP4.NFIL3/E4BP4 expression also plays an important role in the immune system. NFIL3 polymorphisms are associated with inflammatory bowel disease (IBD).

This antibody is a chimeric antibody composed of guinea pig Ig κ and rabbit Fc. Please use anti-rabbit IgG antibody as a secondary antibody.


Monoclonal Antibody of 100 µl targeting NFIL3.

Target: NFIL3
Product Type: Antibody
Size: 100 µl
Application: FCM, IHC, IP, WB
Research Area / Disease: Immunology, Neuroscience
Conjugate: Unlabeled
Antibody Type: Monoclonal
Clone Number: 42
Isotype: IgG ĸ
Immunogen: Recombinant protein, corresponding to internal region of mouse Nfil3 (E4bp4)
Species Reactivity: Human, Mouse
Source: Purified IgG from transfectant
Gene ID Human:


Gene ID Mouse:


Storage Temperature: -20°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.


  1. Yu F et al. The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition. Theranostics. 9, 2754-2767 (2019)(PMID:31244920)


  1. Yu X et al. Th17 cell differentiation is regulated by the circadian clock. Science. 342, 727-30 (2013)(PMID: 24202171
  2. Firth MA et al. Nfil3-independent lineage maintenance and antiviral response of natural killer cells. J Exp Med. 210, 2981-90 (2013)
    (PMID: 24277151
  3. Kashiwada M et al. IL-4-induced transcription factor NFIL3/E4BP4 controls IgE class switching. PNAS 107, 821-6 (2010)(PMID: 20080759
  4. Mitsui S et al. Antagonistic role of E4BP4 and PAR proteins in the circadian oscillatory mechanism. Genes Dev. 15, 995-1006 (2001)(PMID: 11316793
  5. Ikushima S et al. Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytes. PNAS 94, 2609-14 (1997)(PMID: 9122243
  6. Zhang W et al. Molecular cloning and characterization of NF-IL3A, a transcriptional activator of the human interleukin-3 promoter. Mol Cell Biol. 15, 6055–63 (1995)(PMID: 7565758
  7. Cowell IG et al. Transcriptional repression by a novel member of the bZIP family of transcription factors. Mol Cell Biol. 12, 3070-7 (1992)(PMID: 1620116
Code M225-3
Product Type Antibody
Size 100 µl
Application FCM, IHC, IP, WB