Anti-LC3 (Human) mAb (Monoclonal Antibody)

LC3 Monoclonal Antibody.

Specifications:

Description

Monoclonal Antibody of 100 µl targeting LC3 for WB.

Target: LC3
Product Type: Antibody
Size: 100 µl
Application: WB
Research Area / Disease: Autophagy
Conjugate: Unlabeled
Antibody Type: Monoclonal
Clone Number: 8E10
Concentration: 1 mg/mL
Formulation: 1 mg/mL in PBS containing 50% Glycerol (pH 7.2). No preservative is contained.
Isotype: IgG2a ĸ
Immunogen: Human LC3 (MAP1LC3B), 1-120 aa (recombinant)
Host Species: Mouse
Species Reactivity: Hamster, Human, Mouse, Rat
Source: Purified IgG from hybridoma supernatant
Reactivity: This clone reacts with LC3B and does not cross-react with LC3A, LC3C, GATE-16 and GABARAP
Gene ID Human:

81631

Gene ID Mouse:

67443

Gene ID Rat:

64862

Storage Temperature: '-20°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Citations

  1. Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med. 19, 1478-88 (2013),
  2. Mukai R, Ohshima T. HTLV-1 HBZ positively regulates the mTOR signaling pathway via inhibition of GADD34 activity in the cytoplasm. Oncogene. (2013) In press.,
  3. Margariti A et al. XBP1 mRNA splicing triggers an autophagic response in endothelial cells through BECLIN-1 transcriptional activation. J Biol Chem 288, 859-72 (2013)
  4. Mukai, R., and Ohshima, T., Oncogene (2013) In press.
  5. Maejima, Y., et al., Nat Med. 19, 1478-1488 (2013) [WB]
  6. Margariti, A., et al., J Biol Chem. 288, 859-872 (2013) [WB]
  7. Kozako T et al. Novel small molecule SIRT2 inhibitors induce cell death in leukemic cell lines. BMC Cancer. 18, 791 (2018)
  8. Saito T et al. An alternative mitophagy pathway mediated by Rab9 protects the heart against ischemia. J Clin Invest. 129, 802-819 (2019)rn
  9. Slowicka K et al. Physical and functional interaction between A20 and ATG16L1-WD40 domain in the control of intestinal homeostasis. Nat Commun. 10, 1834 (2019)

References

  1. Jia, W., et al., J. Immunol. 186, 5313-5322 (2011)
  2. Tabata, K., et al., Mol. Biol. Cell 21, 4162-4172 (2010)
  3. Mookerjee, S., et al., J. Neurosci. 29, 15134-15144 (2009)
  4. Saitoh, T., et al., Nature 456, 264-268 (2008)
  5. Kabeya, Y., et al., J. Cell Sci. 117, 2805-2812 (2004)
  6. Mizushima, N., et al., Mol. Biol. Cell 15, 1101-1111 (2004)
  7. Mizushima, N., et al., J. Cell Biol. 152, 657-668 (2001)
  8. Schmitz, J., et al., Immunity 23, 479-490 (2005)