Anti-LC3 (Human) mAb

  • Applications
    • WB
  • Target LC3
  • Host Species Mouse
  • Species Reactivities Hamster, Human, Mouse, Rat
  • Code # M186-3
  • Size 100 μl
  • Price
    $341.75
Specifications

Alternative Names

microtubule-associated protein 1 light chain 3 alpha, MAP1BLC3, MAP1ALC3, LC3, LC3A, ATG8E

Background

Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3.
  • Antibody Type:
    Monoclonal
  • Application:
    WB
  • Clone Number:
    80000000000
  • Concentration:
    1 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal Antibody of 100 µl targeting LC3 for WB.

  • Formulation:
    1 mg/mL in PBS containing 50% Glycerol (pH 7.2). No preservative is contained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Gene ID (Rat):
  • Host Species:
    Mouse
  • Immunogen:
    Human LC3 (MAP1LC3B), 1-120 aa (recombinant)
  • Isotype:
    IgG2a ĸ
  • Product Type:
    Antibody
  • Reactivity:
    This clone reacts with LC3B and does not cross-react with LC3A, LC3C, GATE-16 and GABARAP
  • Research Area:
    Autophagy
  • Short Description:

    LC3 Monoclonal Antibody.

  • Size:
    100 μl
  • Species Reactivity:
    Hamster, Human, Mouse, Rat
  • Storage Temperature:
    -20°C
  • Target:
    LC3
Citations
  1. Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med. 19, 1478-88 (2013),
  2. Mukai R, Ohshima T. HTLV-1 HBZ positively regulates the mTOR signaling pathway via inhibition of GADD34 activity in the cytoplasm. Oncogene. (2013) In press.,
  3. Margariti A et al. XBP1 mRNA splicing triggers an autophagic response in endothelial cells through BECLIN-1 transcriptional activation. J Biol Chem 288, 859-72 (2013)
  4. Mukai, R., and Ohshima, T., Oncogene (2013) In press.
  5. Maejima, Y., et al., Nat Med. 19, 1478-1488 (2013) [WB]
  6. Margariti, A., et al., J Biol Chem. 288, 859-872 (2013) [WB]
References
  1. Jia, W., et al., J. Immunol. 186, 5313-5322 (2011)
  2. Tabata, K., et al., Mol. Biol. Cell 21, 4162-4172 (2010)
  3. Mookerjee, S., et al., J. Neurosci. 29, 15134-15144 (2009)
  4. Saitoh, T., et al., Nature 456, 264-268 (2008)
  5. Kabeya, Y., et al., J. Cell Sci. 117, 2805-2812 (2004)
  6. Mizushima, N., et al., Mol. Biol. Cell 15, 1101-1111 (2004)
  7. Mizushima, N., et al., J. Cell Biol. 152, 657-668 (2001)
  8. Schmitz, J., et al., Immunity 23, 479-490 (2005)