Anti-p62 (SQSTM1) (Human) mAb

  • Applications
    • FCM
    • ICC
    • IHC
    • IP
    • WB
  • Target p62
  • Host Species Mouse
  • Species Reactivities Human
  • Code # M162-3
  • Size 100 µl
  • Price
    $243.29
Specifications

Alternative Names

sequestosome 1, p60 p62, A170, OSIL, PDB3, ZIP3, p62

Background

p62/SQSTM1 interacts with various molecular groups such as RIP, TRAF6, ERK, aPKCs, and poly-ubiquitin through PB1 domain, Zn finger domain, and UBA domain. This protein directly interacts with LC3, which is localized on autophagosome membrane, and is degradated by autophagic-lysosome pathway. p62 regulates ubiquitin-positive protein aggregates caused by autophagy deficiency.
  • Antibody Type:
    Monoclonal
  • Application:
    FCM, ICC, IHC, IP, WB
  • Clone Number:
    5F2
  • Concentration:
    1 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal Antibody of 100 µl targeting p62 for FCM, ICC, IHC, IP, WB.

  • Formulation:
    100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Mouse
  • Immunogen:
    Recombinant human p62 (120-440 a.a.)
  • Isotype:
    IgG1 ĸ
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with human p62 on Western blotting, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
  • Research Area:
    Autophagy
  • Short Description:

    p62 Monoclonal Antibody.

  • Size:
    100 µl
  • Species Reactivity:
    Human
  • Storage Temperature:
    -20°C
  • Target:
    p62
Citations
  1. Akaishi R et al. Autophagy in the placenta of women with hypertensive disorders in pregnancy. Placenta 35, 974-80 (2014),
  2. Fan W et al. Keap1 facilitates p62-mediated ubiquitin aggregate clearance via autophagy. Autophagy 6, 614-21 (2010),
  3. Guo X et al. Patulin induces pro-survival functions via autophagy inhibition and p62 accumulation. Cell Death Dis. 4, e822 (2013),
  4. Janda E et al. Parkinsonian toxin-induced oxidative stress inhibits basal autophagy in astrocytes via NQO2/quinone oxidoreductase 2: Implications for neuroprotection. Autophagy 11, 1063-80 (2015),
  5. Matsumoto G et al. Serine 403 phosphorylation of p62/SQSTM1 regulates selective autophagic clearance of ubiquitinated proteins. Mol Cell. 44, 279-89 (2011),
  6. Onodera Y et al. NRF2 immunolocalization in human breast cancer patients as a prognostic factor. Endocr Relat Cancer. 21, 241-52 (2014),
  7. Park JM et al. Prognostic impact of Beclin 1, p62/sequestosome 1 and LC3 protein expression in colon carcinomas from patients receiving 5-fluorouracil as adjuvant chemotherapy. Cancer Biol Ther. 14, 100-7 (2013),
  8. Rovetta F et al. ER signaling regulation drives the switch between autophagy and apoptosis in NRK-52E cells exposed to cisplatin. Exp Cell Res. 318, 238-50 (2012),
  9. Schmeisser H et al. Type I interferons induce autophagy in certain human cancer cell lines. Autophagy 9, 683-96 (2013),
  10. Soll C et al. Serotonin promotes tumor growth in human hepatocellular cancer. Hepatology 51, 1244-54 (2010),
  11. Wang BS et al. Autophagy negatively regulates cancer cell proliferation via selectively targeting VPRBP. Clin Sci (Lond). 124, 203-14 (2013),
  12. Zhu X et al. Cytosolic HMGB1 controls the cellular autophagy/apoptosis checkpoint during inflammation. J Clin Invest. 125, 1098-110 (2015)
References
  1. Ichimura, Y., et al., J. Biol. Chem. 283, 22847-22857 (2008)
  2. Komatsu, M., et al., Cell 131, 1149-1163 (2007)