Anti-pro-IL-18 (Clone 43A11) (Human) mAb

  • Applications
    • WB
  • Target IL-18
  • Host Species Mouse
  • Species Reactivities Human, Mouse
  • Code # M156-3
  • Size 100 μg
  • Price
    $311.90
Specifications

Alternative Names

interleukin 18 (interferon-γ-inducing factor), IGIF, IL1F4, IL-1g, IL-18

Background

Interleukin 18 (IL-18) is an 18-kDa cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24-kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN-γ also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 have also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells.
  • Antibody Type:
    Monoclonal
  • Application:
    WB
  • Clone Number:
    43A11
  • Concentration:
    1 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal Antibody of 100 μg targeting IL-18 for WB.

  • Formulation:
    100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Mouse
  • Immunogen:
    Human pro-IL-18N-terminal peptide(10-36 aa)
  • Isotype:
    IgG1 ĸ
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with human andmouse pro-IL-18 on Western blotting.
  • Research Area:
    Immunology
  • Short Description:

    IL-18 Monoclonal Antibody.

  • Size:
    100 μg
  • Species Reactivity:
    Human, Mouse
  • Storage Temperature:
    -20°C
  • Target:
    IL-18
References
  1. Gu, Y., et al., Science 275, 206-209 (1997)
  2. Ghayur, T., et al., Nature 386, 619-623 (1997)
  3. Dao, T., et al., Cell Immunol. 173, 230-235 (1996)
  4. Micallef, M., et al., Eur. J. Immunol. 26, 1647-1651 (1996)
  5. Ushio, S., et al., J. Immunol. 156, 4274-4279 (1996)
  6. Okamura, H., et al., Nature 378, 88-91 (1995)