Anti-Atg12 (Human) mAb

  • Applications
    • ICC
    • IP
    • WB
  • Target Atg12
  • Host Species Mouse
  • Species Reactivities Human
  • Code # M154-3
  • Size 100 µL
  • Price
    $324.38
Specifications

Alternative Names

autophagy related 12, APG12, FBR93, APG12L,HAPG12

Background

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Autophagy has two ubiquitin-like conjugation systems, the Atg12 and LC3-II systems. In the Atg12 conjugation system, the Atg12-Atg5-Atg16L forms 800 kDa complex that elongates autophagic isolation membrane. After completion of the formation of the autophagosome, the Atg12-Atg5-Atg16L complex dissociates from the membrane.
  • Antibody Type:
    Monoclonal
  • Application:
    ICC, IP, WB
  • Clone Number:
    600000
  • Concentration:
    1 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal Antibody of 100 µl targeting Atg12 for ICC, IP, WB.

  • Formulation:
    100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Mouse
  • Immunogen:
    KLH-conjugated synthetic peptide (Human Atg12, internal reagion)
  • Isotype:
    IgG1 ĸ
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with humanAtg5-Atg12 complex (55 kDa) on Western blotting.Because almost all Atg12 exist in the form of Atg5-Atg12complex, it is difficult to detect the monomeric Atg12.
  • Research Area:
    Autophagy
  • Short Description:

    Atg12 Monoclonal Antibody.

  • Size:
    100 µL
  • Species Reactivity:
    Human
  • Storage Temperature:
    -20°C
  • Target:
    Atg12
References
  1. Mizushima, N., et al., J. Cell Sci. 116, 1679-1688 (2003)
  2. Mizushima, N., et al., FEBS Lett. 532, 450-454 (2002)
  3. Tanida, I., et al., J. Biol. Chem. 276, 1701-1706 (2001)