Anti-Atg5

Atg5 Monoclonal Antibody.

Specifications:

Description

Monoclonal Antibody of 100 μg targeting Atg5 for WB.

Target: Atg5
Product Type: Antibody
Size: 100 μg
Application: WB
Research Area / Disease: Autophagy
Conjugate: Unlabeled
Antibody Type: Monoclonal
Clone Number: 4D3
Concentration: 1 mg/mL
Formulation: 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Isotype: IgG1 ĸ
Immunogen: Full-length recombinant human Atg5 (275 a.a.)
Host Species: Mouse
Species Reactivity: Hamster, Human, Mouse
Source: This antibody was purified from hybridoma (clone 4D3) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with recombinant full-length human Atg5 (275 aa).
Reactivity: This antibody reacts with Atg5-Atg12 complex (55 kDa) on Western blotting.
Gene ID Human:

9474

Gene ID Mouse:

11793

Storage Temperature: -20°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Citations

  1. Takaesu G, et al., TGF?A-activated kinase 1 (TAK1)-binding proteins (TAB) 2 and 3 negatively regulate autophagy. J Biochem. 151, 157-166 (2012),
  2. Takahashi Y, et al., Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy. Autophagy. 7, 61?73 (2011),
  3. Young MM, et al., Autophagosomal membrane serves as platform for intracellular death-inducing signaling complex (iDISC)-mediated caspase-8 activation and apoptosis. J Biol Chem. 287, 12455-12468 (2012)
  4. Young, M., et al., J. Biol. Chem. in press
  5. Takaesu, G., et al., J. Biochem. 151, 157-166 (2012)
  6. Takahashi, Y., et al., Autophagy 7, 61-73 (2011)
  7. Scrivo A et al. Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production. Nat Commun. 10, 780 (2019)
  8. Zhang, Huazhi, et al. “RNF186 regulates EFNB1 (ephrin B1)-EPHB2-induced autophagy in the colonic epithelial cells for the maintenance of intestinal homeostasis.” Autophagy 17.10 (2021): 3030-3047.
  9. Wu, Shan-Ying, et al. “Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo.” Autophagy (2022): 1-19.
  10. Lee, Kyung Hye, et al. “Role of ATP5G3 in sodium nitroprusside‐induced cell death in cervical carcinoma cells.” Journal of Biochemical and Molecular Toxicology (2022): e23267.

References

  1. Hanada, T., et al., J. Biol. Chem. 282, 37298-37302 (2007)
  2. Pyo, J. O., et al., J. Biol. Chem. 280, 20722-20729 (2005)
  3. Mizushima, N., et al., J. Cell Biol. 152, 657-667 (2001)