Anti-Atg5
Atg5 Monoclonal Antibody.
Specifications:
Description
Monoclonal Antibody of 100 μg targeting Atg5 for WB.
| Target: | Atg5 |
|---|---|
| Product Type: | Antibody |
| Size: | 100 μg |
| Application: | WB |
| Research Area / Disease: | Autophagy |
| Conjugate: | Unlabeled |
| Antibody Type: | Monoclonal |
| Clone Number: | 4D3 |
| Concentration: | 1 mg/mL |
| Formulation: | 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Isotype: | IgG1 ĸ |
| Immunogen: | Full-length recombinant human Atg5 (275 a.a.) |
| Host Species: | Mouse |
| Species Reactivity: | Hamster, Human, Mouse |
| Source: | This antibody was purified from hybridoma (clone 4D3) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with recombinant full-length human Atg5 (275 aa). |
| Reactivity: | This antibody reacts with Atg5-Atg12 complex (55 kDa) on Western blotting. |
| Gene ID Human: | |
| Gene ID Mouse: | |
| Storage Temperature: | -20°C |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
Citations
- Takaesu G, et al., TGF?A-activated kinase 1 (TAK1)-binding proteins (TAB) 2 and 3 negatively regulate autophagy. J Biochem. 151, 157-166 (2012),
- Takahashi Y, et al., Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy. Autophagy. 7, 61?73 (2011),
- Young MM, et al., Autophagosomal membrane serves as platform for intracellular death-inducing signaling complex (iDISC)-mediated caspase-8 activation and apoptosis. J Biol Chem. 287, 12455-12468 (2012)
- Young, M., et al., J. Biol. Chem. in press
- Takaesu, G., et al., J. Biochem. 151, 157-166 (2012)
- Takahashi, Y., et al., Autophagy 7, 61-73 (2011)
- Scrivo A et al. Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production. Nat Commun. 10, 780 (2019)
- Zhang, Huazhi, et al. “RNF186 regulates EFNB1 (ephrin B1)-EPHB2-induced autophagy in the colonic epithelial cells for the maintenance of intestinal homeostasis.” Autophagy 17.10 (2021): 3030-3047.
- Wu, Shan-Ying, et al. “Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo.” Autophagy (2022): 1-19.
- Lee, Kyung Hye, et al. “Role of ATP5G3 in sodium nitroprusside‐induced cell death in cervical carcinoma cells.” Journal of Biochemical and Molecular Toxicology (2022): e23267.
References
- Hanada, T., et al., J. Biol. Chem. 282, 37298-37302 (2007)
- Pyo, J. O., et al., J. Biol. Chem. 280, 20722-20729 (2005)
- Mizushima, N., et al., J. Cell Biol. 152, 657-667 (2001)
Related Content:
Brochures
Autophagy-Related Products Catalog
