Anti-LC3 (Human) mAb

  • Applications
    • FCM
    • ICC
    • IP
    • WB
  • Target LC3
  • Host Species Mouse
  • Species Reactivities Hamster, Human, Mouse, Rat
  • Code # M152-3
  • Size 200 μg
  • Price
    $359.13
Specifications

Alternative Names

microtubule-associated protein 1 light chain 3 beta, microtubule-associated protein 1 light chain 3 alpha, LC3B, ATG8F, MAP1LC3B-a, MAP1A, 1BLC3, MAP1LC3B, LC3A, ATG8E, MAP1ALC3, MAP1BLC3, MAP1LC3A

Background

Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3.
  • Antibody Type:
    Monoclonal
  • Application:
    FCM, ICC, IP, WB
  • Clone Number:
    4E+12
  • Concentration:
    2 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    This LC3 antibody is validated for multiple applications (WB, ICC, IP, FCM) and has over 25 citations. This is a monoclonal antibody of 200 μg targeting LC3 for FCM, ICC, IP, WB.

    More information about LC3 and autophagy can be found in our Blog. In addition you may download our Autophagy Pathway Poster.

  • Formulation:
    200 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Gene ID (Rat):
  • Host Species:
    Mouse
  • Immunogen:
    Recombinant human LC3 (MAP1LC3B: 1-120 a.a.)
  • Isotype:
    IgG1 ĸ
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with LC3 (MAP1LC3A, B) on Immunocytochemistry, Western blotting and Immunoprecipitation.
  • Research Area:
    Autophagy
  • Short Description:

    LC3 Monoclonal Antibody.

  • Size:
    200 μg
  • Species Reactivity:
    Hamster, Human, Mouse, Rat
  • Storage Temperature:
    -20°C
  • Target:
    LC3
Citations
  1. Cai T et al. Deletion of Ia-2 and/or Ia-2?A in mice decreases insulin secretion by reducing the number of dense core vesicles. Diabetologia. 54, 2347-57 (2011),
  2. Chalazonitis A et al. Homeodomain interacting protein kinase 2 regulates postnatal development of enteric dopaminergic neurons and glia via BMP signaling. J Neurosci. 31, 13746-57 (2011),
  3. Eng KE et al. A novel quantitative flow cytometry-based assay for autophagy. Autophagy. 6, 634-41 (2010),
  4. Frankel LB et al. microRNA-101 is a potent inhibitor of autophagy. EMBO J. 30, 4628-41 (2011),
  5. Gillis JM et al. Aminopeptidase-resistant peptides are targeted to lysosomes and subsequently degraded. Traffic. 12, 1897-910 (2011),
  6. Hartleben B et al. Autophagy influences glomerular disease susceptibility and maintains podocyte homeostasis in aging mice. J Clin Invest. 120, 1084-96 (2010),
  7. Ho H et al. WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition. J Biol Chem. 286, 12509-23 (2011),
  8. Joubert PE et al. Chikungunya virus-induced autophagy delays caspase-dependent cell death. J Exp Med. 209,1029-47 (2012),
  9. Kovacs JR et al. Autophagy promotes T-cell survival through degradation of proteins of the cell death machinery. Cell Death Differ. 19, 144-52 (2012),
  10. McKnight NC et al. Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC. EMBO J. 31, 1931-46 (2012),
  11. Moreau K et al. Autophagosome precursor maturation requires homotypic fusion. Cell. 146, 303-17 (2011),
  12. Morris CR et al. A knockout of the Tsg101 gene leads to decreased expression of ErbB receptor tyrosine kinases and induction of autophagy prior to cell death. PLoS One. 7, e34308 (2012),
  13. Saiki S et al. Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition. Autophagy. 7, 176-87 (2011),
  14. Seto S et al. Coronin-1a inhibits autophagosome formation around Mycobacterium tuberculosis-containing phagosomes and assists mycobacterial survival in macrophages. Cell Microbiol. 14, 710-27 (2012),
  15. Shi CS et al. Activation of autophagy by inflammatory signals limits IL-1?A production by targeting ubiquitinated inflammasomes for destruction. Nat Immunol. 13, 255-63 (2012),
  16. Sims JJ et al. Polyubiquitin-sensor proteins reveal localization and linkage-type dependence of cellular ubiquitin signaling. Nat Methods. 9, 303-9 (2012),
  17. Starr T et al. Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle. Cell Host Microbe. 11, 33-45 (2012),
  18. Thayanidhi N et al. Alpha-synuclein delays endoplasmic reticulum (ER)-to-Golgi transport in mammalian cells by antagonizing ER/Golgi SNAREs. Mol Biol Cell. 21, 1850-63 (2010),
  19. Traver MK et al. Immunity-related GTPase M (IRGM) proteins influence the localization of guanylate-binding protein 2 (GBP2) by modulating macroautophagy. J Biol Chem. 286, 30471-80 (2011),
  20. Wang Z et al. Autophagy regulates myeloid cell differentiation by p62/SQSTM1-mediated degradation of PML-RARα oncoprotein. Autophagy. 7, 401-11 (2011)
  21. Ho, H., et al., J. Biol. Chem. 286, 12509-12523 (2011) [WB]
  22. Saiki, S., et al., Autophagy 7, 176-187 (2011) [WB]
  23. Kasai, M., et al., J. Immunol. 183, 7278-7285 (2009) [WB, IC, IHC]
  24. Mookerjee, S., et al., J. Neurosci. 29, 15134-15144 (2009) [IP, IHC]
  25. Nishitsuji, K., et al., Am. J. Pathol. 174, 957-969 (2009) [IC]
  26. Wang, Y., et al., J. Biol. Chem. 283, 4766-4777 (2008) [WB, IC]
References
  1. Kabeya, Y., et al., J. Cell Sci. 117, 2805-2812 (2004)
  2. Mizushima, N., et al., Mol. Biol. Cell 15, 1101-1111 (2004)
  3. Mizushima, N., et al., J. Cell Biol. 152, 657-667 (2001)
  4. Kabeya, Y., et al., EMBO J. 19, 5720-5728 (2000)