Anti-monomeric Kusabira-Green N-terminal fragment mAb

  • Applications
    • WB
  • Target Kusabira-Green
  • Host Species Mouse
  • Code # M148-3M
  • Size 100 µL
  • Price
    $751.91
Specifications

Background

CoralHue® Fluo-chase Kit can detect protein-protein interactions as fluorescent signals using the protein fragment complementation method. The gene of CoralHue® monomeric Kusabira-Green (mKG), a reporter protein, is divided into two fragments (CoralHue® mKG_N fragment and CoralHue®mKG_C fragment) which are respectively fused to the target protein genes to investigate the interactions. When the expressed target proteins don’t interact, CoralHue®mKG_N fragment and CoralHue®mKG_C fragment can not approach each other and can not emit fluorescence. However, when target proteins interact, divided CoralHue®mKG fragments spatially approach each other and the local effective concentration increases. As a result, mKG fragments form a steric structure before dividing and the chromophore emits fluorescence. The fluorescent signals can be detected depending on the fused target protein-protein interactions. Clone 1E6 has the epitope on CoralHue®mKG_N fragment and can detect the fusion protein with CoralHue®mKG_N specifically.
  • Antibody Type:
    Monoclonal
  • Application:
    WB
  • Clone Number:
    1E6
  • Concentration:
    1 mg/mL
  • Conjugate:
    Unlabeled
  • Formulation:
    100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  • Host Species:
    Mouse
  • Immunogen:
    mKG_N (1-168 a.a.)
  • Isotype:
    IgG2b
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with CoralHue® mKG_N fragment and CoralHue® mKO1 and CoralHue® mKO2 on Western blotting.
  • Research Area:
    Fluorescent Proteins
  • Size:
    100 µL
  • Storage Temperature:
    -20°C
  • Target:
    Kusabira-Green
Citations
  1. Nitta S et al. Hepatitis C virus NS4B protein targets STING and abrogates RIG-I-mediated type I interferon-dependent innate immunity. Hepatology. 57: 46-58. 2013