Anti-p53 (Human) mAb

  • Applications
    • IHC
    • IP
    • WB
  • Target p53
  • Host Species Mouse
  • Species Reactivities Human
  • Code # K0181-3
  • Size 100 μg
  • Price
    $233.93
Specifications

Background

The tumor suppressor p53 controls numerous downstream targets that can result in variable outcomes, including apoptosis, transient growth arrest, and sustained growth arrest or senescence. p53 is induced on DNA damage to prevent gene amplification and preserve genetic stability. Induction of G1 arrest is dependent upon sequence-specific DNA binding and transcriptional activation of p53 target genes such as p21WAF-1/CIP-1. p21 is an inhibitor of cyclin dependent kinase (CDK) and blocks the activity of G1 cyclin-CDK complexes. In cells induction of p21 can block phosphorylation and inactivation of the retinoblastoma protein, thus contributing to G1 arrest. p53 functions to eliminate and inhibit the proliferation of abnormal cells, thereby preventing neoplastic development. Abrogation of the negative growth regulatory functions of p53 occurs in many, perhaps all, human tumours.
  • Antibody Type:
    Monoclonal
  • Application:
    IHC, IP, WB
  • Clone Number:
    DO-1
  • Concentration:
    1 mg/mL
  • Conjugate:
    Unlabeled
  • Description:

    Monoclonal Antibody of 100 μg targeting p53 for IHC, IPP, WB.

  • Formulation:
    100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
  • Gene ID (Human):
  • Gene ID (Mouse):
  • Host Species:
    Mouse
  • Immunogen:
    N-terminal synthetic peptide of human p53 (11-25 aa)
  • Isotype:
    IgG2a
  • Product Type:
    Antibody
  • Reactivity:
    This antibody reacts with human p53.
  • Research Area:
    Apoptosis
  • Short Description:

    p53 Monoclonal Antibody.

  • Size:
    100 μg
  • Species Reactivity:
    Human
  • Storage Temperature:
    -20°C
  • Target:
    p53
References
  1. Lohmann, DR., et al., Cancer Res. 53, 5797-5801 (1993)
  2. Hansen, S., et al., J. Biol. Chem. 271, 3917-3924 (1996)
  3. Hsiao, M., et al., Biochem. Biophys. Res. Commun. 233,
  4. 359-364 (1997)
  5. Brázda, V., et al., Biochem. Biophys. Res. Commun. 267,
  6. 934-939 (2000)
  7. Sun, L., et al., J. Biol. Chem. 286, 4226-4235 (2011)