Anti-CD8a (Human) mAb-FITC (Monoclonal Antibody)

DATA SHEET

Description
The Hit8a monoclonal antibody reacts with the human CD8a molecule, a 32 kDa cell surface receptor expressed either as a heterodimer (CD8 α/β) or as a homodimer (CD8 α/α) on the majority of thymocytes, a subpopulation of mature T cells, and natural killer cells. CD8 interacts with the major histocompatibility complex class I (MHC class I) molecules on antigen presenting cells or epithelial cells. The Hit8a antibody reacts with 13-48% of peripheral lymphocytes, 80% of thymocytes, and a subset of natural killer cells.

HIT8a, RPA-T8, and OKT8 antibodies do not compete with each other for binding to peripheral leukocytes, meaning that that they do not recognize the same epitope or block each other by steric hindrance.

Preparation & Storage
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Application Notes
The antibody has been analyzed for quality through the flow cytometric analysis of the relevant cell type. The antibody can be used at less than or  equal to 5 μL per test. A test is the amount of antibody required to stain a cell sample in the final volume of 100 μL.