ExoCap™ Ultracentrifugation/Storage Booster (ExoCap™ USB)
- Improve the recovery rate of exosomes from cell culture supernatant using ultracentrifugation
- Increase stability using low temperature storage of purified exosomes
Exosomes and microvesicles are submicron extracellular vesicles that are secreted by various cell types. These vesicles contain various proteins, nucleic acids and molecular constituents to contribute to cell:cell communications. Currently, there are many different methods to collect exosomes. Ultracentrifugation is the most common isolation method used by most researchers. However, this method does have drawbacks, including reduced recovery ratio in cell culture supernatant. Another shortcoming involves storage temperature for isolated exosomes. Currently, storage is not stable at 4°C, which is a disadvantage for many researchers who may want to research the exosomes at a later time.
ExoCap™ Ultracentrifugation / Storage Booster reagent is available for efficient exosome purification by ultracentrifugation. It can be used as an additive to improve the recovery rate of exosomes in cultured supernatant. Furthermore, it can improve the storage stability of purified exosome under low temperature conditions.
Procedure Summary for ExoCap™ Ultracentrifugation / Storage Booster (ExoCap™ USB)
Evaluation of the ExoCap USB stability by Western blotting (Non-reduced)
Exosomes were isolated from the supernatant by ultracentrifugation with/without ExoCap. The intensity of the bands of exosome markers with the ExoCap USB were denser than those without ExoCap USB, as a robust signal is seen in Lane 2 for each marker. Exosome recovery was boosted by using the ExoCap USB.
Evaluation of the ExoCap USB recovery by CLE
Exosome recovery rate was improved with the addition of ExoCap USB. For beads, CLEIA (Chemiluminescent Enzyme Immuno Assay) ExoCap™ Streptavidin Kits, biotinylated CD81 antibody and alkaline phosphatase (ALP) labeled CD9 antibody were used.
Stability for 7 days at 4 degree C in regular Polypropylene tube
The effect of the storage stability duration using ExoCap USB was evaluated. Purified exosomes were stored for 7 days at 4°C and evaluated using CLEIA. When ExoCap USB was added, the intensity ratio was higher than those where ExoCap USB was not used for all concentrations.
|MEX-USB||ExoCap Ultracentrifugation/Storage Booster||50 ml||Reagent|