ExoCap™ Streptavidin CD9/CD63/CD81 Set

Reagents for exosome isolation from human-derived samples

  • High-purity exosomes can be isolated by a simple procedure
  • Isolated exosomes can be analyzed by WB, qRT-PCR, CLEIA, ELISA, and FCM

Components

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Biotinylated antibodies to CD9, CD63, and CD81 that are highly specific and perform excellently in exosome isolation by immunoprecipitation.

The ExoCap™ Streptavidin Kit consists of streptavidin-immobilized magnetic beads (Magnosphere™) and buffer solutions for capturing, washing, and extracting exosomes. The Magnosphere™ is coated with a JSR Life Sciences Corporation proprietary hydrophilic polymer to decrease non-specific binding. You can isolate exosomes in high-purity by using it together with our original biotinylated antibodies specific to CD9, CD63, and CD81.

Outline of ExoCap™ Streptavidin CD9/CD63/CD81 Set 

1) Preparation of Capture Beads Mix

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2) Isolation of exosomes

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Western blot analysis of isolated exosomes

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After allowing the biotinylated CD9, CD63, and CD81 antibodies to bind separately to Streptavidin Magnetic Beads, an equal volume of each antibody-bound beads  was mixed together (CD9/CD63/CD81 Mix). Using the mixed beads, exosomes were isolated from each sample and subjected to western blot analysis.

qRT-PCR analysis of miR-21 in exosomes

 exocap

After allowing the biotinylated CD9, CD63, and CD81 antibodies to bind separately to Streptavidin Magnetic Beads, an equal volume of each antibody-bound beads  was mixed together (CD9/CD63/CD81 Mix). Using the mixed beads, exosomes were isolated from each sample. Nucleic acid was purified with ExoCap™ Nucleic Acid Elution Buffer (Code No. MEX-E) and subjected to qRT-PCR to detect miR-21.

Related Product Highlight 

ExoCap™ Streptavidin Kit

  • Flexible use of biotinylated antibodies to target exosomes

ExoCap™ Ultracentrifugation/Storage Booster

  • Improve the recovery rate of exosomes from cell culture supernatant using ultracentrifugation
  • Increase stability using low temperature storage of purified exosomes

Use our selection of biotinylated antibodies as well as other related products with your Exocap™ Streptavidin Kit

NameCloneVolumeProduct TypeIsotypeApplicationCross Reactivity
ExoCap™ Streptavidin Kit - - Exosome Enrich Kit - - -
ExoCap™ Ultracentrifugation/Storage Booster   50 mL Reagent      
ExoCap™ Streptavidin CD9/CD63/CD81 Set     Exosome Enrich Kit      
Anti-CD9 mAb-Biotin A100-4 50 μg/100 μL  Antibody Mouse IgG2a  WB, IP, FCM Hu, (Mky)
Anti-CD63{LAMP-3} mAb-Biotin
C047-1 50 μg/50 μL 
Antibody Mouse IgG2a  WB, FCM  Hu, (Mky)
Anti-CD81 {TAPA1} mAb-Biotin A103-10 50 μg/50 μL Antibody Mouse IgG2a WB, IP, FCM Hu, (Ham, Mky)
Anti-EpCAM mAb-Biotin
B8-4 50 μg/50 μL Antibody Mouse IgG1  FCM Hu
Anti-CD9 mAb A100-4 100 μg/100 μL Antibody Mouse IgG2a WB, IP, FCM Hu, Mky
Anti-CD9 mAb-ALP A100-4 50uL Antibody IgG2a k ELISA Hu
Anti-CD9 mAb-FITC A100-4 50ug/100uL Antibody IgG2a k FCM Hu
Anti-CD63 {LAMP-3} mAb C047-1 100 μg/100 μL Antibody Mouse IgG2a WB, IP, FCM Hu, Mky
Anti-CD63 {LAMP-3} mAb-ALP C047-1 50uL Antibody IgG2a k ELISA Hu
Anti-CD63 {LAMP-3} mAb-FITC C047-1 50ug/100uL Antibody IgG2a k FCM Hu
Anti-CD81 {TAPA1} mAb A103-10 100 μg/100 μL Antibody Mouse IgG2a WB, IP, FCM Hu, Ham, Mky
Anti-CD81 {TAPA1} mAb-ALP A103-10 50uL Antibody IgG2a k ELISA Hu
Anti-CD81 {TAPA1} mAb-FITC A103-10 50ug/100uL Antibody IgG2a k FCM Hu
Mouse IgG1 (isotype control)-Biotin 2E12 50 μg/50 μL Biotinylated mAb  - FCM 
Mouse IgG2a (isotype control)-Biotin 6H3 50 μg/50 μL Biotinylated mAb - FCM -
Mouse IgG2b (isotype control)-Biotin 3D12 50 μg/50 μL Biotinylated mAb - FCM -
Mouse IgG3 (isotype control)-Biotinb 6A3 50 μg/50 μL Biotinylated mAb - FCM -
ExoDiluent for Immunoassay - 50ml - - - -
Protein G-Magnetic Beads - 10ml - - - -
Magnetic Rack  - 1 unit (1.5ml x 8 tube)  -  -
Anti-CD9 (Mouse) mAb JF9 100 µg/100 µL Antibody - FCM -
Anti-CD9 (Mouse) mAb-FITC JF9 50 µg/100 µL Antibody - FCM -
Anti-CD9 (Human) mAb 10H6 100 µg/100 µL Antibody Mouse IgG1 k WB. FCM Hu
Anti-CD-9 (Human) mAb-PE 10H6 1mL Antibody  Mouse IgG1 k  FCM Hu
Anti-CD63 (LAMP3) (Mouse) mAb  R5G2 100 µg/100 µL Antibody   Rat IgG2b  WB, FCM, IC* Mo
Anti-EpCAM (CD326) (Mouse) mAb  2-17-F1  100 µL Antibody  Rat IgG2a   FCM Mo
Anti-EIF2C1 (AGO1) pAb  -  200 µL Antibody   Rabbit Ig (aff.)  WB, IP, RIP  Hu, Mo
Anti-EIF2C2 (AGO2) pAb  -  100 µg/100 µL Antibody   Rabbit Ig (aff.)  WB  Hu, Mo, Rat
Anti-TNRC6A (GW182) (Human) pAb  -  200 µg/200 µL Antibody   Rabbit Ig (aff.)  WB, IP, IC, RIP  Hu
Anti-FBL (Fibrillarin) pAb  -  100 µL: 1 mg/mL Antibody   Rabbit Ig (aff.)  WB, IP  Hu, Mo, Rat, Ham
Anti-DCP1B (Human) pAb  -  100 µg/100 µL Antibody  Rabbit Ig (aff.)  WB, IP  Hu

 














































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