Epitope Tag Purification Kits and Gels

Purification Kit

This kit is optimized to purify target epitope-tagged proteins without any loss of protein activity or denaturation.

MBLI's tagged Protein PURIFICATION KIT employs competitive polypeptides to elute target tagged proteins under a neutral pH to maintain target protein function and conformation. Epitope tag specific antibodies in this kit are highly optimized for target recovery with high yield and purity from crude samples like cell lysates or culture supernatant of mammalian transfectant cells. 

PurificationKit1 

This product is a set of purification gel, elution peptide, wash buffer, and mini column

 

 

 

 

 

 

Procedure Summary

PurificationKit2

  

Tagged Protein Magnetic Purification Kits

PurificationKit1

 

  • Contains magnet-conjugated antibodies, elution peptide, and wash solutions
  • Recommended for purifying small amounts of sample 
  • No need for centrifugation
  • High yield
  • Maintain target protein function and conformation

 

 

 

 

 

Procedure Summary

PurificationKit

c-Myc-tagged GST protein purification

PurificationKit

 
V5-tagged β-galactosidase purification

PurificationKit

 

Purified in native conformations

Maintain target protein function and conformation under a neutral pH condition using MBLI's purification procedure. This procedure is useful for pull down assay as well as analysis of co-immunoprecipitated proteins.

Purification and enzymatic activity of N-terminal His-tagged β-galactosidase

PurificationKit3

Pull-down assay

PurificationKit4

 

High Purity

Recover high-purity tagged proteins by using our original proprietary antibodies that have superior affinity and specificity to the epitope tag.

Purification of C-terminal His-tagged protein X from culture supernatant.

PurificationKit5

 

PURIFICATION GEL with Elution Peptide

PurificationKit1 

Recover high-purity tagged proteins by using our original proprietary antibodies that have superior affinity and specificity to the epitope tag.

 

 
 
 
 
 
Purification of C-terminal His-tagged protein X from culture supernatant

PurificationKit7

 

Purification Gel

PurificationKit1 

This product consists of a purification gel only.

 
 
 
 
 
 
 
 
 
Purification of N-terminal DDDDK-tagged β-galactosidase

PurificationKit8

 

Purification Cartridge 

PurificationKit1 

*Ready-to-use-cartridge for the isolation of c-Myc-tagged
*The cartridge can be used directly on automated AKTA or FPLC systems. (Cartridge is 1/16 inch female end)

 

 

 

PurificationKit