Anti-5-methylcytidine (m<sup>5</sup>C) mAb

  • Applications
    • ELISA
    • ICC
    • IHC
    • IP
    • WB
  • Target 5-methylcytidine (m5C)
  • Host Species Mouse
  • Code # D346-3
  • Size 100 µl
  • Price
    $328.61
Specifications

Alternative Names

m5Cyd

Background

5-Methylcytosine (5mC), a modified nucleobase derived from cytosine, is present in both DNA and RNA molecules. 5mC in DNA is an essential epigenetic mark associated with gene silencing and genome stability during embryonic development. 5mC in ribonucleoside, called 5-methylcytidine (m5C), is involved in structural stability, aminoacylation and codon recognition of tRNA. In rRNA, m5C affects translational fidelity and tRNA recognition. m5C-modified nucleobase also have been identified in mRNA and other non-coding RNA but the biological functions are still unclear. m5C is detected not only in tissues and cells but also in urine. It is reported that the levels of m5C change in urine or tissues from patients with various diseases such as bladder, breast and colon cancers.
  • Antibody Type:
    Monoclonal
  • Application:
    ELISA, ICC, IHC, IP, WB
  • Clone Number:
    FMC-9
  • Conjugate:
    Unlabeled
  • Description:
    Monoclonal antibody of 100 µl targeting 5-methylcytidine m5C for WB, IPP, ICC, IHC, ELISA.
  • Formulation:
    1 mg/mL in PBS/50% glycerol, pH 7.2
  • Host Species:
    Mouse
  • Immunogen:
    KLH-conjugated 5-methylcytidine (KLH-m5C)
  • Isotype:
    IgG2a λ
  • Product Type:
    Antibody
  • Research Area:
    Epigenetics
  • Short Description:
    5-methylcytidine m5C Monoclonal Antibody.
  • Size:
    100 µl
  • Storage Temperature:
    -20°C
  • Target:
    5-methylcytidine (m5C)
Citations
  1. Mishima E et al. Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. PLoS One 10, e0143756 (2015)
References
  1. Seki, Y., et al., Dev. Biol. 278, 440-458 (2005)
  2. Sakai, Y., et al., Cell Struct. Funct. 26, 685-691 (2001) [IHC-fr]
  3. Mizugaki, M., et al., Biol. Pharm. Bull. 19, 1537-1540 (1996)