Anti-Mutated IDH1/2 (Human) mAb

  • Applications
    • ELISA
    • WB
  • Target Mutated IDH1/2 (Human)
  • Host Species Rat
  • Species Reactivities Human
  • Code # D332-3
  • Size 100 µl
  • Price
    $328.61
Specifications

Alternative Names

isocitrate dehydrogenase 1 (NADP+), soluble isocitrate dehydrogenase 2 (NADP+), mitochondrial IDH IDP IDCD IDPC PICD HEL-216 HEL-S-26 IDHM IDPM ICD-M D2HGA2 mNADP-IDH

Background

Isocitrate dehydrogenase 1 (IDH1) mutations, which have been identified as early and frequent genetic alterations in astrocytomas, oligodendrogliomas, and oligoastrocytomas, as well as in secondary glioblastomas, might be the initiating event in these glioma subtypes. In contrast, primary glioblastomas, as well as other systemic cancers, rarely contain IDH1 mutations. The IDH1 mutations are remarkably specific to a single codon in the conserved and functionally important Arginine 132 residue (R132) in IDH1. Mutations in the analogous IDH2-R172 codon also occur at a lower rate in these cancers. IDH1/2 mutations were observed subsequently in 22% of acute myeloid leukemias. IDH1 mutations were found to result in the ability of the enzyme to catalyze the reduced NADP-dependent reduction of α-ketoglutarate to R(-)-2-hydroxyglutarate (2-HG). Reduction of α-ketoglutarate by 2-HG or mutant IDH1 results in a lower level of prolyl hydroxylases and promotes the accumulation of hypoxia-inducible factor (HIF) 1α. HIF-1α levels were greater in human gliomas harboring an IDH1 mutation than in tumors without the mutation. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.
  • Antibody Type:
    Monoclonal
  • Application:
    ELISA, WB
  • Clone Number:
    MsMab-2
  • Conjugate:
    Unlabeled
  • Description:
    Monoclonal antibody of 100 µl targeting Mutated IDH1/2 (Human) for WB, ELISA.
  • Formulation:
    1 mg/mL in PBS containing 50% glycerol. No preservative is contained.
  • Gene ID (Human):
  • Host Species:
    Rat
  • Isotype:
    IgG2a ĸ
  • Product Type:
    Antibody
  • Research Area:
    Cancer
  • Short Description:
    Mutated IDH1/2 (Human) Monoclonal Antibody.
  • Size:
    100 µl
  • Species Reactivity:
    Human
  • Storage Temperature:
    -20°C
  • Target:
    Mutated IDH1/2 (Human)
Citations
  1. Liu X et al. Isocitrate dehydrogenase 2 mutation is a frequent event in osteosarcoma detected by a multi-specific monoclonal antibody MsMab-1. Cancer Med. 2, 803-814 (2013),
  2. Ogasawara S et al. Multi-specific monoclonal antibody MsMab-2 recognizes IDH1-R132L and IDH2-R172M mutations. Monoclon. Antib. Immunodiagn. Immunother., 32, 377-381 (2013)
References
  1. Liu, X., et al., Cancer Med. 2, 803-814 (2013) [WB]
  2. Ogasawara, S., et al., Monoclon.Antib.Immunodiagn.Immunother. 32, 377-381 (2013) [WB, ELISA]