Anti-His-tag mAb (Monoclonal Antibody)

  • Applications
    • FCM
    • ICC
    • IP
    • WB
  • Target His
  • Host Species Mouse
  • Code # D291-3B
  • Size 10µl
  • Price


The His-tag (6xHis-tag) is one of the most common tags used to facilitate the purification of recombinant proteins. Metal chelate affinity chromatography is widely used for purification of His-tagged proteins. This specific antibody is useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of the recombinant proteins.
  • Antibody Type:
  • Application:
    FCM, ICC, IP, WB
  • Clone Number:
  • Concentration:
    1 mg/mL
  • Description:
    Monoclonal antibody of 50 µl targeting His-tag for FCM, ICC, IPP, WB.
  • Host Species:
  • Immunogen:
    6xHis-tagged protein
  • Isotype:
    IgG2a ĸ
  • Product Type:
  • Reactivity:
    This antibody recognizes His-tagged protein on Western blotting, Immunoprecipitation, Immunocytochemistry and Flow cytometry.
  • Research Area:
    Cell Biology
  • Size:
  • Storage Temperature:
  • Target:
  1. Kawada J et al. Heteroclitic serological response in esophageal and prostate cancer patients after NY-ESO-1 protein vaccination. Int J Cancer 130, 584-92 (2012) (PMID:21413013)
  2. Suzuki T et al. Monoubiquitination of Tob/BTG family proteins competes with degradation-targeting polyubiquitination. Biochem Biophys Res Commun. 409, 70-4 (2011) (PMID:21549103)
  3. Li T et al. RNF168-mediated H2A neddylation antagonizes its ubiquitination and regulates DNA damage repair. J Cell Sci. (2014) In press. (PMID:24634510)
  4. Hu B et al. HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation. Nucleic Acids Res. 42, 5582-93 (2014) (PMID:24711370)