Anti-His-tag mAb (Monoclonal Antibody)

His-tag Monoclonal Antibody.

Specifications:

Description

Monoclonal antibody of 200 µl targeting His-tag for FCM, ICC, IPP, WB.

Target: His
Product Type: Antibody
Size: 200 μl
Application: FCM, ICC, IP, WB
Research Area / Disease: Epitope Tag
Conjugate: Unlabeled
Antibody Type: Monoclonal
Clone Number: OGHis
Concentration: 1 mg/mL
Formulation: 200 μg IgG in 200 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
Isotype: IgG2a ĸ
Immunogen: 6xHis-tagged protein
Host Species: Mouse
Source: This antibody was purified from hybridoma(clone OGHis) supernatant using protein A agarose. Thishybridoma was established by fusion of mouse myelomacell SP-1 with Balb/c mouse splenocyte immunized with6xHis tagged protein.
Reactivity: This antibody recognizes His-taggedprotein on Western blotting, Immunoprecipitation,Immunocytochemistry and Flow cytometry.
Storage Temperature: '-20°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Citations

  1. Hu B et al. HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation. Nucleic Acids Res. 42, 5582-93 (2014),
  2. Kawada J et al. Heteroclitic serological response in esophageal and prostate cancer patients after NY-ESO-1 protein vaccination. Int J Cancer 130, 584-92 (2012),
  3. Li T et al. RNF168-mediated H2A neddylation antagonizes its ubiquitination and regulates DNA damage repair. J Cell Sci. (2014) In press.,
  4. Suzuki T et al. Monoubiquitination of Tob/BTG family proteins competes with degradation-targeting polyubiquitination. Biochem Biophys Res Commun. 409, 70-4 (2011)
    1. Cannavo E et al. Regulatory control of DNA end resection by Sae2 phosphorylation. Nat Commun. 9, 4016 (2018)
    2. Yang C et al. Ribosomal protein L6 (RPL6) is recruited to DNA damage sites in a poly(ADP-ribose) polymerase-dependent manner and regulates the DNA damage response. J Biol Chem. 294, 2827-2838 (2019)
    3. Yamagishi Aet al. The Structural Function of Nestin in Cell Body Softening is Correlated with Cancer Cell Metastasis. Int J Biol Sci. 15, 1546-1556 (2019)
    4. Lu X et al. GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival. Nucleic Acids Res. 47, 10977-10993 (2019)
    5. An W et al. Glucocorticoid modulatory element-binding protein 1 (GMEB1) interacts with the de-ubiquitinase USP40 to stabilize CFLARL and inhibit apoptosis in human non-small cell lung cancer cells. J Exp Clin Cancer Res. 38, 181 (2019)
    6. Hidayat M  et al.  Role of FBXW7 in the quiescence of gefitinib-resistant lung cancer stem cells in EGFR-mutant non-small cell lung cancer. Bosn J Basic Med Sci. 19, 355-367 (2019)

    References

    1. Kawada J et al. Heteroclitic serological response in esophageal and prostate cancer patients after NY-ESO-1 protein vaccination. Int J Cancer 130, 584-92 (2012) (PMID:21413013)
    2. Suzuki T et al. Monoubiquitination of Tob/BTG family proteins competes with degradation-targeting polyubiquitination. Biochem Biophys Res Commun. 409, 70-4 (2011) (PMID:21549103)
    3. Li T et al. RNF168-mediated H2A neddylation antagonizes its ubiquitination and regulates DNA damage repair. J Cell Sci. (2014) In press. (PMID:24634510)
    4. Hu B et al. HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation. Nucleic Acids Res. 42, 5582-93 (2014) (PMID:24711370)
    Code D291-3
    Product Type Antibody
    Size 200 μl
    Application FCM, ICC, IP, WB
    Price
    $328.61
    Qty: